FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963666708> ?p ?o ?g. }

• W1963666708 endingPage "357" @default.
• W1963666708 startingPage "350" @default.
• W1963666708 abstract "Lyme disease is the fastest-growing zoonotic disease in North America. Current methods for detection of Borrelia burgdorferi infection are challenged by analysis subjectivity and standardization of antigen source. In the present study, we developed an immuno-PCR (iPCR)-based approach employing recombinant in vivo-expressed B. burgdorferi antigens for objective detection of a host immune response to B. burgdorferi infection. iPCR is a liquid-phase protein detection method that combines the sensitivity of PCR with the specificity and versatility of immunoassay-based protocols. Use of magnetic beads coated with intact spirochetes provided effective antigen presentation and allowed detection of host-generated antibodies in experimentally infected mice at day 11 postinoculation, whereas host-generated antibodies were detected at day 14 by enzyme-linked immunosorbent assay (ELISA) and day 21 by immunoblotting. Furthermore, magnetic beads coated with recombinant B. burgdorferi in vivo-expressed antigen OspC or BmpA demonstrated positive detection of host-generated antibodies in mice at day 7 postinoculation with markedly increased iPCR signals above the background, with the quantification cycle (C(q)) value for each sample minus the mean background C(q) plus 3 standard deviations (ΔC(q)) being 4 to 10, whereas ΔC(q) was 2.5 for intact spirochete-coated beads. iPCR demonstrated a strong correlation (Spearman rank correlation = 0.895, P < 0.0001) with a commercial ELISA for detection of host antibodies in human Lyme disease patient sera using the B. burgdorferi VlsE C6 peptide. In addition, iPCR showed potential applicability for direct detection of spirochetes in blood. The results presented here indicate that our iPCR assay has the potential to provide an objective format that can be used for sensitive detection of multiple host response antibodies and isotypes to B. burgdorferi infection." @default.
• W1963666708 created "2016-06-24" @default.
• W1963666708 creator A5013912332 @default.
• W1963666708 creator A5057391523 @default.
• W1963666708 creator A5065097421 @default.
• W1963666708 date "2013-01-09" @default.
• W1963666708 modified "2023-09-29" @default.
• W1963666708 title "Enhanced Detection of Host Response Antibodies to Borrelia burgdorferi Using Immuno-PCR" @default.
• W1963666708 cites W1994800091 @default.
• W1963666708 cites W2002993469 @default.
• W1963666708 cites W2006813546 @default.
• W1963666708 cites W2014537967 @default.
• W1963666708 cites W2033077259 @default.
• W1963666708 cites W2050105290 @default.
• W1963666708 cites W2053139562 @default.
• W1963666708 cites W2061848805 @default.
• W1963666708 cites W2064634177 @default.
• W1963666708 cites W2084272002 @default.
• W1963666708 cites W2094386668 @default.
• W1963666708 cites W2103128190 @default.
• W1963666708 cites W2111485154 @default.
• W1963666708 cites W2111626612 @default.
• W1963666708 cites W2119411841 @default.
• W1963666708 cites W2125641599 @default.
• W1963666708 cites W2127269466 @default.
• W1963666708 cites W2134630348 @default.
• W1963666708 cites W2138906694 @default.
• W1963666708 cites W2139104181 @default.
• W1963666708 cites W2143591164 @default.
• W1963666708 cites W2147279763 @default.
• W1963666708 cites W2149023602 @default.
• W1963666708 cites W2156758916 @default.
• W1963666708 cites W2160725896 @default.
• W1963666708 cites W2162689245 @default.
• W1963666708 cites W2164581303 @default.
• W1963666708 cites W2165149106 @default.
• W1963666708 cites W2168950809 @default.
• W1963666708 cites W2169541824 @default.
• W1963666708 cites W4230991986 @default.
• W1963666708 cites W4236013903 @default.
• W1963666708 doi "https://doi.org/10.1128/cvi.00630-12" @default.
• W1963666708 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3592355" @default.
• W1963666708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23302740" @default.
• W1963666708 hasPublicationYear "2013" @default.
• W1963666708 type Work @default.
• W1963666708 sameAs 1963666708 @default.
• W1963666708 citedByCount "30" @default.
• W1963666708 countsByYear W19636667082013 @default.
• W1963666708 countsByYear W19636667082014 @default.
• W1963666708 countsByYear W19636667082015 @default.
• W1963666708 countsByYear W19636667082016 @default.
• W1963666708 countsByYear W19636667082017 @default.
• W1963666708 countsByYear W19636667082018 @default.
• W1963666708 countsByYear W19636667082019 @default.
• W1963666708 countsByYear W19636667082020 @default.
• W1963666708 countsByYear W19636667082021 @default.
• W1963666708 countsByYear W19636667082022 @default.
• W1963666708 countsByYear W19636667082023 @default.
• W1963666708 crossrefType "journal-article" @default.
• W1963666708 hasAuthorship W1963666708A5013912332 @default.
• W1963666708 hasAuthorship W1963666708A5057391523 @default.
• W1963666708 hasAuthorship W1963666708A5065097421 @default.
• W1963666708 hasBestOaLocation W19636667081 @default.
• W1963666708 hasConcept C104317684 @default.
• W1963666708 hasConcept C147483822 @default.
• W1963666708 hasConcept C159047783 @default.
• W1963666708 hasConcept C159654299 @default.
• W1963666708 hasConcept C203014093 @default.
• W1963666708 hasConcept C2777553296 @default.
• W1963666708 hasConcept C2779159148 @default.
• W1963666708 hasConcept C2780420688 @default.
• W1963666708 hasConcept C40767141 @default.
• W1963666708 hasConcept C55493867 @default.
• W1963666708 hasConcept C86803240 @default.
• W1963666708 hasConcept C89423630 @default.
• W1963666708 hasConceptScore W1963666708C104317684 @default.
• W1963666708 hasConceptScore W1963666708C147483822 @default.
• W1963666708 hasConceptScore W1963666708C159047783 @default.
• W1963666708 hasConceptScore W1963666708C159654299 @default.
• W1963666708 hasConceptScore W1963666708C203014093 @default.
• W1963666708 hasConceptScore W1963666708C2777553296 @default.
• W1963666708 hasConceptScore W1963666708C2779159148 @default.
• W1963666708 hasConceptScore W1963666708C2780420688 @default.
• W1963666708 hasConceptScore W1963666708C40767141 @default.
• W1963666708 hasConceptScore W1963666708C55493867 @default.
• W1963666708 hasConceptScore W1963666708C86803240 @default.
• W1963666708 hasConceptScore W1963666708C89423630 @default.
• W1963666708 hasIssue "3" @default.
• W1963666708 hasLocation W19636667081 @default.
• W1963666708 hasLocation W19636667082 @default.
• W1963666708 hasLocation W19636667083 @default.
• W1963666708 hasLocation W19636667084 @default.
• W1963666708 hasLocation W19636667085 @default.
• W1963666708 hasOpenAccess W1963666708 @default.
• W1963666708 hasPrimaryLocation W19636667081 @default.
• W1963666708 hasRelatedWork W1977902082 @default.
• W1963666708 hasRelatedWork W2005022263 @default.
• W1963666708 hasRelatedWork W2022178101 @default.

• next