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- W1963701812 abstract "The possibility of enhancing glutathione peroxidase (GPx) activity and cytosolic total antioxidant activity (TAA) in normoxia and hypoxia/reoxygenation (H/R) by the supplementation of different concentrations of sodium selenite (SS) or selenomethionine (SM) was investigated in cultured rat cardiomyocytes. To assess the entity of oxidative stress due to H/R, levels of conjugated dienes containing lipids were determined. In normoxia, GPx activity and TAA increased in parallel with the increase in SS and SM supplementation. H/R did not influence GPx activity but lowered TAA; both SS and SM supplementations were effective in increasing GPx activity, the most effective concentration being 1 μM. At this SS and SM concentration, TAA returned to a normoxic value. Conjugated diene production, increased by H/R, was reduced by SS and SM supplementation, the 1 μM concentration appearing to be the most effective one. According to these data Se supplementation represents another possibility to counteract oxidative damage in the myocardium. Keywords: Selenium; hypoxia/reoxygenation; cultured cardiomyocytes; glutathione peroxidase; oxidative stress" @default.
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- W1963701812 date "2003-01-21" @default.
- W1963701812 modified "2023-09-25" @default.
- W1963701812 title "Selenium Supplementation Can Protect Cultured Rat Cardiomyocytes from Hypoxia/Reoxygenation Damage" @default.
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- W1963701812 doi "https://doi.org/10.1021/jf026017d" @default.
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