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- W1963702011 abstract "A highly sensitive and specific liquid chromatographic procedure with peroxyoxalate chemiluminescence detection has been developed for the determination of fluocortin butyl, a 3 alpha-ketocorticosteroid, in blood plasma. The technique employs dansylation of the steroid to provide a highly chemiluminescent derivative. After separation by reversed-phase liquid chromatography, reagents necessary for chemiluminescence are added, followed by detection in a conventional fluorimetric detector in which the excitation source is deactivated. The precision is 2.5% relative standard deviation at the 10 ng/ml level, and the response is linear up to at least 4 ng injected steroid. The procedure requires only 1 ml blood plasma and has a limit of detection of 100 pg/ml or 7.5 pg injected steroid. The system is reliably used for routine pharmacokinetic studies and with modifications, is applicable to other steroids as well." @default.
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- W1963702011 date "1984-12-01" @default.
- W1963702011 modified "2023-09-26" @default.
- W1963702011 title "Determination of trace levels of steroids in blood plasma by liquid chromatography with peroxyoxalate chemiluminescence detection" @default.
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- W1963702011 doi "https://doi.org/10.1016/s0021-9673(01)91675-9" @default.
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