FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963707717> ?p ?o ?g. }

• W1963707717 endingPage "8276" @default.
• W1963707717 startingPage "8269" @default.
• W1963707717 abstract "Proteins respond to electrostatic perturbations through complex reorganizations of their charged and polar groups, as well as those of the surrounding media. These solvation responses occur both in the protein interior and on its surface, though the exact mechanisms of solvation are not well understood, in part because of limited data on the solvation responses for any given protein. Here, we characterize the solvation kinetics at sites throughout the sequence of a small globular protein, the B1 domain of streptococcal protein G (GB1), using the synthetic fluorescent amino acid Aladan. Aladan was incorporated into seven different GB1 sites, and the time-dependent Stokes shift was measured over the femtosecond to nanosecond time scales by fluorescence upconversion and time-correlated single photon counting. The seven sites range from buried within the protein core to fully solvent-exposed on the protein surface, and are located on different protein secondary structures including β-sheets, helices, and loops. The dynamics in the protein sites were compared against the free fluorophore in buffer. All protein sites exhibited an initial, ultrafast Stokes shift on the subpicosecond time scale similar to that observed for the free fluorophore, but smaller in magnitude. As the probe is moved from the surface to more buried sites, the dynamics of the solvation response become slower, while no clear correlation between dynamics and secondary structure is observed. We suggest that restricted movements of the surrounding protein residues give rise to the observed long time dynamics and that such movements comprise a large portion of the protein's solvation response. The proper treatment of dynamic Stokes shift data when the time scale for solvation is comparable to the fluorescence lifetime is discussed." @default.
• W1963707717 created "2016-06-24" @default.
• W1963707717 creator A5007451401 @default.
• W1963707717 creator A5019490617 @default.
• W1963707717 creator A5055295290 @default.
• W1963707717 creator A5059853775 @default.
• W1963707717 creator A5061536008 @default.
• W1963707717 creator A5087362230 @default.
• W1963707717 date "2007-06-26" @default.
• W1963707717 modified "2023-10-10" @default.
• W1963707717 title "Measurement of Solvation Responses at Multiple Sites in a Globular Protein" @default.
• W1963707717 cites W1965031981 @default.
• W1963707717 cites W1966354786 @default.
• W1963707717 cites W1966600252 @default.
• W1963707717 cites W1967097202 @default.
• W1963707717 cites W1969026917 @default.
• W1963707717 cites W1969192381 @default.
• W1963707717 cites W1970432336 @default.
• W1963707717 cites W1976984447 @default.
• W1963707717 cites W1986918874 @default.
• W1963707717 cites W1994673901 @default.
• W1963707717 cites W1998481913 @default.
• W1963707717 cites W1999840987 @default.
• W1963707717 cites W2010301288 @default.
• W1963707717 cites W2013939950 @default.
• W1963707717 cites W2014583061 @default.
• W1963707717 cites W2020601552 @default.
• W1963707717 cites W2020716464 @default.
• W1963707717 cites W2022440554 @default.
• W1963707717 cites W2023690254 @default.
• W1963707717 cites W2026678187 @default.
• W1963707717 cites W2026907550 @default.
• W1963707717 cites W2027175366 @default.
• W1963707717 cites W2040301369 @default.
• W1963707717 cites W2040351161 @default.
• W1963707717 cites W2046515692 @default.
• W1963707717 cites W2047253280 @default.
• W1963707717 cites W2047475161 @default.
• W1963707717 cites W2050622376 @default.
• W1963707717 cites W2051397621 @default.
• W1963707717 cites W2054947925 @default.
• W1963707717 cites W2055442639 @default.
• W1963707717 cites W2056692623 @default.
• W1963707717 cites W2057742133 @default.
• W1963707717 cites W2058011467 @default.
• W1963707717 cites W2060595131 @default.
• W1963707717 cites W2060858171 @default.
• W1963707717 cites W2063675340 @default.
• W1963707717 cites W2067012477 @default.
• W1963707717 cites W2069891688 @default.
• W1963707717 cites W2072043602 @default.
• W1963707717 cites W2086862822 @default.
• W1963707717 cites W2090065141 @default.
• W1963707717 cites W2094117242 @default.
• W1963707717 cites W2094441820 @default.
• W1963707717 cites W2095134060 @default.
• W1963707717 cites W2103389503 @default.
• W1963707717 cites W2106390970 @default.
• W1963707717 cites W2108407770 @default.
• W1963707717 cites W2110726459 @default.
• W1963707717 cites W2119988927 @default.
• W1963707717 cites W2120153949 @default.
• W1963707717 cites W2128277122 @default.
• W1963707717 cites W2132524280 @default.
• W1963707717 cites W2139982366 @default.
• W1963707717 cites W2149316939 @default.
• W1963707717 cites W2149413451 @default.
• W1963707717 cites W2160988970 @default.
• W1963707717 cites W2162832464 @default.
• W1963707717 cites W2322059529 @default.
• W1963707717 doi "https://doi.org/10.1021/jp0709104" @default.
• W1963707717 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2507720" @default.
• W1963707717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17592867" @default.
• W1963707717 hasPublicationYear "2007" @default.
• W1963707717 type Work @default.
• W1963707717 sameAs 1963707717 @default.
• W1963707717 citedByCount "101" @default.
• W1963707717 countsByYear W19637077172012 @default.
• W1963707717 countsByYear W19637077172013 @default.
• W1963707717 countsByYear W19637077172014 @default.
• W1963707717 countsByYear W19637077172015 @default.
• W1963707717 countsByYear W19637077172016 @default.
• W1963707717 countsByYear W19637077172017 @default.
• W1963707717 countsByYear W19637077172018 @default.
• W1963707717 countsByYear W19637077172019 @default.
• W1963707717 countsByYear W19637077172020 @default.
• W1963707717 countsByYear W19637077172021 @default.
• W1963707717 countsByYear W19637077172022 @default.
• W1963707717 countsByYear W19637077172023 @default.
• W1963707717 crossrefType "journal-article" @default.
• W1963707717 hasAuthorship W1963707717A5007451401 @default.
• W1963707717 hasAuthorship W1963707717A5019490617 @default.
• W1963707717 hasAuthorship W1963707717A5055295290 @default.
• W1963707717 hasAuthorship W1963707717A5059853775 @default.
• W1963707717 hasAuthorship W1963707717A5061536008 @default.
• W1963707717 hasAuthorship W1963707717A5087362230 @default.
• W1963707717 hasBestOaLocation W19637077172 @default.
• W1963707717 hasConcept C105168689 @default.

• next