Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963711243> ?p ?o ?g. }
- W1963711243 endingPage "e79" @default.
- W1963711243 startingPage "e79" @default.
- W1963711243 abstract "Bacteria react to adverse environmental stimuli by clustering into organized communities called biofilms. A remarkably sophisticated control system based on the dinucleotide 3′–5′ cyclic diguanylic acid (c-di-GMP) is involved in deciding whether to form or abandon biofilms. The ability of c-di-GMP to form self-intercalated dimers is also thought to play a role in this complex regulation. A great advantage in the quest of elucidating the catalytic properties of the enzymes involved in c-di-GMP turnover (diguanylate cyclases and phosphodiesterases) would come from the availability of an experimental approach for in vitro quantification of c-di-GMP in real-time. Here, we show that c-di-GMP can be detected and quantified by circular dichroism (CD) spectroscopy in the low micromolar range. The method is based on the selective ability of manganese ions to induce formation of the intercalated dimer of the c-di-GMP dinucleotide in solution, which displays an intense sigmoidal CD spectrum in the near-ultraviolet region. This characteristic spectrum originates from the stacking interaction of the four mutually intercalated guanines, as it is absent in the other cyclic dinucleotide 3′–5′ cyclic adenilic acid (c-di-AMP). Thus, near-ultraviolet CD can be used to effectively quantify in real-time the activity of diguanylate cyclases and phosphodiesterases in solution." @default.
- W1963711243 created "2016-06-24" @default.
- W1963711243 creator A5005422954 @default.
- W1963711243 creator A5009944462 @default.
- W1963711243 creator A5011714668 @default.
- W1963711243 creator A5055728648 @default.
- W1963711243 creator A5062209793 @default.
- W1963711243 creator A5062217195 @default.
- W1963711243 creator A5068959451 @default.
- W1963711243 creator A5088523485 @default.
- W1963711243 creator A5088748370 @default.
- W1963711243 date "2013-01-28" @default.
- W1963711243 modified "2023-10-16" @default.
- W1963711243 title "Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy" @default.
- W1963711243 cites W1966857950 @default.
- W1963711243 cites W1967562956 @default.
- W1963711243 cites W1969635440 @default.
- W1963711243 cites W1971007680 @default.
- W1963711243 cites W1976347829 @default.
- W1963711243 cites W1980822406 @default.
- W1963711243 cites W1990657825 @default.
- W1963711243 cites W1991102091 @default.
- W1963711243 cites W1991658240 @default.
- W1963711243 cites W1997444893 @default.
- W1963711243 cites W1999422525 @default.
- W1963711243 cites W2000835389 @default.
- W1963711243 cites W2012746341 @default.
- W1963711243 cites W2018508409 @default.
- W1963711243 cites W2019819756 @default.
- W1963711243 cites W2026704710 @default.
- W1963711243 cites W2027142684 @default.
- W1963711243 cites W2032294516 @default.
- W1963711243 cites W2036195365 @default.
- W1963711243 cites W2046520603 @default.
- W1963711243 cites W2070804022 @default.
- W1963711243 cites W2078749450 @default.
- W1963711243 cites W2079462554 @default.
- W1963711243 cites W2082159746 @default.
- W1963711243 cites W2086323657 @default.
- W1963711243 cites W2091895129 @default.
- W1963711243 cites W2092263123 @default.
- W1963711243 cites W2092760470 @default.
- W1963711243 cites W2094144273 @default.
- W1963711243 cites W2103828045 @default.
- W1963711243 cites W2105972088 @default.
- W1963711243 cites W2112584669 @default.
- W1963711243 cites W2118194237 @default.
- W1963711243 cites W2122394318 @default.
- W1963711243 cites W2130257593 @default.
- W1963711243 cites W2131350133 @default.
- W1963711243 cites W2149138461 @default.
- W1963711243 cites W2151044396 @default.
- W1963711243 cites W2151490567 @default.
- W1963711243 cites W2153047034 @default.
- W1963711243 cites W2156062569 @default.
- W1963711243 cites W2160682526 @default.
- W1963711243 cites W2160765784 @default.
- W1963711243 cites W2161257009 @default.
- W1963711243 cites W2168421163 @default.
- W1963711243 cites W2323618083 @default.
- W1963711243 cites W2323747692 @default.
- W1963711243 doi "https://doi.org/10.1093/nar/gkt028" @default.
- W1963711243 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3627566" @default.
- W1963711243 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23358823" @default.
- W1963711243 hasPublicationYear "2013" @default.
- W1963711243 type Work @default.
- W1963711243 sameAs 1963711243 @default.
- W1963711243 citedByCount "43" @default.
- W1963711243 countsByYear W19637112432013 @default.
- W1963711243 countsByYear W19637112432014 @default.
- W1963711243 countsByYear W19637112432015 @default.
- W1963711243 countsByYear W19637112432016 @default.
- W1963711243 countsByYear W19637112432017 @default.
- W1963711243 countsByYear W19637112432018 @default.
- W1963711243 countsByYear W19637112432019 @default.
- W1963711243 countsByYear W19637112432020 @default.
- W1963711243 countsByYear W19637112432021 @default.
- W1963711243 countsByYear W19637112432022 @default.
- W1963711243 countsByYear W19637112432023 @default.
- W1963711243 crossrefType "journal-article" @default.
- W1963711243 hasAuthorship W1963711243A5005422954 @default.
- W1963711243 hasAuthorship W1963711243A5009944462 @default.
- W1963711243 hasAuthorship W1963711243A5011714668 @default.
- W1963711243 hasAuthorship W1963711243A5055728648 @default.
- W1963711243 hasAuthorship W1963711243A5062209793 @default.
- W1963711243 hasAuthorship W1963711243A5062217195 @default.
- W1963711243 hasAuthorship W1963711243A5068959451 @default.
- W1963711243 hasAuthorship W1963711243A5088523485 @default.
- W1963711243 hasAuthorship W1963711243A5088748370 @default.
- W1963711243 hasBestOaLocation W19637112431 @default.
- W1963711243 hasConcept C12554922 @default.
- W1963711243 hasConcept C178790620 @default.
- W1963711243 hasConcept C181199279 @default.
- W1963711243 hasConcept C185592680 @default.
- W1963711243 hasConcept C2776043855 @default.
- W1963711243 hasConcept C2779546866 @default.
- W1963711243 hasConcept C55493867 @default.
- W1963711243 hasConcept C62826618 @default.