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- W1963722658 abstract "A series of luciferase reporter constructs was prepared from a 1035‐bp fragment of mouse genomic DNA flanking the 5′‐coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full‐length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site‐directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2 ." @default.
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- W1963722658 date "2006-10-19" @default.
- W1963722658 modified "2023-10-13" @default.
- W1963722658 title "Functional characterization of the promoter for the mouse<i>SPTLC2</i>gene, which encodes subunit 2 of serine palmitoyltransferase" @default.
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- W1963722658 doi "https://doi.org/10.1016/j.febslet.2006.10.025" @default.
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