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• W1963732118 abstract "The high-affinity GABA transporter in neurons and glial cells is the primary means of inactivating synaptic GABA. In the present study, a rat GABA transporter (GAT-1)-specific probe was used to quantitate GAT-1 mRNA in cultured neurons and glial cells from rat brain. GAT-1 mRNA is expressed in neurons but not in pure cultures of astrocytes. Incubation of neurons with forskolin led to concentration- and time-dependent decreases in GAT-1 mRNA. This effect could be also achieved by chronic exposure of neurons to 8-Br-cAMP and dib-cAMP but not with 1,9-dideoxyforskolin. This effect on the levels of GAT-1 mRNA correlates with a decrease in the Na(+)-dependent GABA transport activity in neurons. Treatment with agents that increase cellular levels of cAMP did not affect GABA transport or GAT-1 mRNA expression in glial cells." @default.
• W1963732118 created "2016-06-24" @default.
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• W1963732118 date "1994-01-01" @default.
• W1963732118 modified "2023-10-13" @default.
• W1963732118 title "Cellular distribution and regulation by cAMP of the GABA transporter (GAT-1) mRNA" @default.
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• W1963732118 doi "https://doi.org/10.1016/0169-328x(94)90387-5" @default.
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