Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963750603> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W1963750603 endingPage "2750" @default.
- W1963750603 startingPage "2746" @default.
- W1963750603 abstract "An appropriate method was developed for the continuous assay of sucrose synthase (SS) (EC 2.4.1.13) by spectrophotometry. The uridine 5‘-diphosphate derived from sucrose synthesis was stoichiometrically coupled to oxidation of β-nicotinamide adenine dinucleotide by the enzymes nucleoside-5‘-diphosphate kinase (NDPK), pyruvate kinase, and lactate dehydrogenase. Utilization of crude extracts led to a complete masking of SS assay by adenylate kinase, adenosine 5‘-triphosphatase (ATPase), and phosphoenolpyruvate phosphatase found in the crude extracts. These interfering enzymes were mostly removed from the crude extracts by using a combination of gel filtration, centrifugation through a selectively permeable membrane (Biomax-100 Ultrafree centrifugal device), and inhibition by the addition of K2HPO4 to the assay buffer. Sensitivity of the SS assay was significantly increased by the inclusion of NDPK and ATP, which are essential to the reaction in the coupling system. Keywords: Sugars; enzyme assay; microfiltration; coupling reactions" @default.
- W1963750603 created "2016-06-24" @default.
- W1963750603 creator A5018417005 @default.
- W1963750603 creator A5067045374 @default.
- W1963750603 creator A5076472215 @default.
- W1963750603 date "1999-06-02" @default.
- W1963750603 modified "2023-09-23" @default.
- W1963750603 title "A Continuous Method for Enzymatic Assay of Sucrose Synthase in the Synthetic Direction" @default.
- W1963750603 cites W1976231664 @default.
- W1963750603 cites W1997782736 @default.
- W1963750603 cites W1999924205 @default.
- W1963750603 cites W2010533159 @default.
- W1963750603 cites W2012279918 @default.
- W1963750603 cites W2016784549 @default.
- W1963750603 cites W2043959950 @default.
- W1963750603 cites W2052367816 @default.
- W1963750603 cites W2055463271 @default.
- W1963750603 cites W2070535837 @default.
- W1963750603 cites W2075286646 @default.
- W1963750603 cites W2085499523 @default.
- W1963750603 cites W2149634239 @default.
- W1963750603 cites W2418254563 @default.
- W1963750603 cites W3149483726 @default.
- W1963750603 doi "https://doi.org/10.1021/jf981264w" @default.
- W1963750603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10552558" @default.
- W1963750603 hasPublicationYear "1999" @default.
- W1963750603 type Work @default.
- W1963750603 sameAs 1963750603 @default.
- W1963750603 citedByCount "6" @default.
- W1963750603 countsByYear W19637506032016 @default.
- W1963750603 crossrefType "journal-article" @default.
- W1963750603 hasAuthorship W1963750603A5018417005 @default.
- W1963750603 hasAuthorship W1963750603A5067045374 @default.
- W1963750603 hasAuthorship W1963750603A5076472215 @default.
- W1963750603 hasConcept C143937172 @default.
- W1963750603 hasConcept C180526460 @default.
- W1963750603 hasConcept C181199279 @default.
- W1963750603 hasConcept C185592680 @default.
- W1963750603 hasConcept C20251656 @default.
- W1963750603 hasConcept C23265538 @default.
- W1963750603 hasConcept C2776901170 @default.
- W1963750603 hasConcept C2778597767 @default.
- W1963750603 hasConcept C2779564974 @default.
- W1963750603 hasConcept C2780854706 @default.
- W1963750603 hasConcept C43617362 @default.
- W1963750603 hasConcept C55493867 @default.
- W1963750603 hasConcept C75520062 @default.
- W1963750603 hasConceptScore W1963750603C143937172 @default.
- W1963750603 hasConceptScore W1963750603C180526460 @default.
- W1963750603 hasConceptScore W1963750603C181199279 @default.
- W1963750603 hasConceptScore W1963750603C185592680 @default.
- W1963750603 hasConceptScore W1963750603C20251656 @default.
- W1963750603 hasConceptScore W1963750603C23265538 @default.
- W1963750603 hasConceptScore W1963750603C2776901170 @default.
- W1963750603 hasConceptScore W1963750603C2778597767 @default.
- W1963750603 hasConceptScore W1963750603C2779564974 @default.
- W1963750603 hasConceptScore W1963750603C2780854706 @default.
- W1963750603 hasConceptScore W1963750603C43617362 @default.
- W1963750603 hasConceptScore W1963750603C55493867 @default.
- W1963750603 hasConceptScore W1963750603C75520062 @default.
- W1963750603 hasIssue "7" @default.
- W1963750603 hasLocation W19637506031 @default.
- W1963750603 hasLocation W19637506032 @default.
- W1963750603 hasOpenAccess W1963750603 @default.
- W1963750603 hasPrimaryLocation W19637506031 @default.
- W1963750603 hasRelatedWork W1198271203 @default.
- W1963750603 hasRelatedWork W1963750603 @default.
- W1963750603 hasRelatedWork W2030034783 @default.
- W1963750603 hasRelatedWork W2040741921 @default.
- W1963750603 hasRelatedWork W2057389622 @default.
- W1963750603 hasRelatedWork W2062071945 @default.
- W1963750603 hasRelatedWork W2078253814 @default.
- W1963750603 hasRelatedWork W2151402563 @default.
- W1963750603 hasRelatedWork W2309427866 @default.
- W1963750603 hasRelatedWork W2071081434 @default.
- W1963750603 hasVolume "47" @default.
- W1963750603 isParatext "false" @default.
- W1963750603 isRetracted "false" @default.
- W1963750603 magId "1963750603" @default.
- W1963750603 workType "article" @default.