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- W1963753629 abstract "By studying the electron paramagnetic resonance (EPR) signals of QA/−-Fe2+TBTQ− and the oxidised non-haem iron we have found that detergent solubilisation of BBY Photosystem II (PS II) preparations using standard methods, involving either the detergents n-octyl β-d-glucopyranoside (OGP) or n-heptyl β-d-thioglucoside (HTG) at pH 6.0, results in loss of bicarbonate binding. New preparations including a dodecyl maltoside (DM) prepared CP47, CP43, D1, D2, cytochrome b-559 complex are described which at pH 7.5 retain native bicarbonate binding. These preparations provide a new system for studies on the ‘bicarbonate effect’ because bicarbonate depletion can now be achieved without displacement by another anion. They are also a more suitable starting material for the isolation of QA retaining D1/D2 reaction centres because the detrimental changes to the QA binding region are avoided." @default.
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- W1963753629 date "1991-09-01" @default.
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- W1963753629 title "Preparation and characterisation of Photosystem II core particles with and without bound bicarbonate" @default.
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- W1963753629 doi "https://doi.org/10.1016/s0005-2728(05)80123-9" @default.
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