Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963764148> ?p ?o ?g. }
- W1963764148 endingPage "703" @default.
- W1963764148 startingPage "698" @default.
- W1963764148 abstract "We have purified the IL-1 beta converting enzyme from the THP-1 cell line using standard chromatographic techniques and obtained the N-terminal amino acid sequence of this novel protein. After stimulation of THP-1 cells with lipopolysaccharide, hydroxyurea, and silica, the protease was solubilized by multiple freeze/thawing. The protein was purified by ion-exchange chromatography, affinity chromatography on blue agarose, gel filtration, and chromatofocusing. The molecular weight of the protein is approximately 22,000 Da and the pI is between 7.1 and 6.8. The overall yield for this procedure was 16% of the activity found in the initial cell lysates. An antiserum raised against a peptide based on the N-terminus was used to precipitate the protease, confirming our identification of the 22,000-Da protein as the IL-1 beta converting enzyme." @default.
- W1963764148 created "2016-06-24" @default.
- W1963764148 creator A5007305453 @default.
- W1963764148 creator A5009348326 @default.
- W1963764148 creator A5033880708 @default.
- W1963764148 creator A5040769344 @default.
- W1963764148 creator A5041060382 @default.
- W1963764148 creator A5054276418 @default.
- W1963764148 creator A5064159346 @default.
- W1963764148 date "1992-08-01" @default.
- W1963764148 modified "2023-09-26" @default.
- W1963764148 title "Purification of interleukin-1β converting enzyme, the protease that cleaves the interleukin-1β precursor" @default.
- W1963764148 cites W1518143481 @default.
- W1963764148 cites W1533420115 @default.
- W1963764148 cites W1563493034 @default.
- W1963764148 cites W1613736574 @default.
- W1963764148 cites W1932052686 @default.
- W1963764148 cites W1965994467 @default.
- W1963764148 cites W1981163610 @default.
- W1963764148 cites W1989047636 @default.
- W1963764148 cites W2009310436 @default.
- W1963764148 cites W2022961230 @default.
- W1963764148 cites W2031855019 @default.
- W1963764148 cites W2034750698 @default.
- W1963764148 cites W2051658957 @default.
- W1963764148 cites W2078872554 @default.
- W1963764148 cites W2100837269 @default.
- W1963764148 cites W4206163632 @default.
- W1963764148 cites W4293247451 @default.
- W1963764148 doi "https://doi.org/10.1016/0003-9861(92)90629-b" @default.
- W1963764148 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1321594" @default.
- W1963764148 hasPublicationYear "1992" @default.
- W1963764148 type Work @default.
- W1963764148 sameAs 1963764148 @default.
- W1963764148 citedByCount "38" @default.
- W1963764148 countsByYear W19637641482012 @default.
- W1963764148 countsByYear W19637641482013 @default.
- W1963764148 countsByYear W19637641482017 @default.
- W1963764148 countsByYear W19637641482019 @default.
- W1963764148 countsByYear W19637641482022 @default.
- W1963764148 crossrefType "journal-article" @default.
- W1963764148 hasAuthorship W1963764148A5007305453 @default.
- W1963764148 hasAuthorship W1963764148A5009348326 @default.
- W1963764148 hasAuthorship W1963764148A5033880708 @default.
- W1963764148 hasAuthorship W1963764148A5040769344 @default.
- W1963764148 hasAuthorship W1963764148A5041060382 @default.
- W1963764148 hasAuthorship W1963764148A5054276418 @default.
- W1963764148 hasAuthorship W1963764148A5064159346 @default.
- W1963764148 hasConcept C104317684 @default.
- W1963764148 hasConcept C153911025 @default.
- W1963764148 hasConcept C167625842 @default.
- W1963764148 hasConcept C179247698 @default.
- W1963764148 hasConcept C181199279 @default.
- W1963764148 hasConcept C185592680 @default.
- W1963764148 hasConcept C193363816 @default.
- W1963764148 hasConcept C206967267 @default.
- W1963764148 hasConcept C2776714187 @default.
- W1963764148 hasConcept C2776829962 @default.
- W1963764148 hasConcept C2778476535 @default.
- W1963764148 hasConcept C2779281246 @default.
- W1963764148 hasConcept C43617362 @default.
- W1963764148 hasConcept C515207424 @default.
- W1963764148 hasConcept C55493867 @default.
- W1963764148 hasConcept C86803240 @default.
- W1963764148 hasConceptScore W1963764148C104317684 @default.
- W1963764148 hasConceptScore W1963764148C153911025 @default.
- W1963764148 hasConceptScore W1963764148C167625842 @default.
- W1963764148 hasConceptScore W1963764148C179247698 @default.
- W1963764148 hasConceptScore W1963764148C181199279 @default.
- W1963764148 hasConceptScore W1963764148C185592680 @default.
- W1963764148 hasConceptScore W1963764148C193363816 @default.
- W1963764148 hasConceptScore W1963764148C206967267 @default.
- W1963764148 hasConceptScore W1963764148C2776714187 @default.
- W1963764148 hasConceptScore W1963764148C2776829962 @default.
- W1963764148 hasConceptScore W1963764148C2778476535 @default.
- W1963764148 hasConceptScore W1963764148C2779281246 @default.
- W1963764148 hasConceptScore W1963764148C43617362 @default.
- W1963764148 hasConceptScore W1963764148C515207424 @default.
- W1963764148 hasConceptScore W1963764148C55493867 @default.
- W1963764148 hasConceptScore W1963764148C86803240 @default.
- W1963764148 hasIssue "2" @default.
- W1963764148 hasLocation W19637641481 @default.
- W1963764148 hasLocation W19637641482 @default.
- W1963764148 hasOpenAccess W1963764148 @default.
- W1963764148 hasPrimaryLocation W19637641481 @default.
- W1963764148 hasRelatedWork W1600195864 @default.
- W1963764148 hasRelatedWork W1963764148 @default.
- W1963764148 hasRelatedWork W1964747279 @default.
- W1963764148 hasRelatedWork W1976967567 @default.
- W1963764148 hasRelatedWork W1988739595 @default.
- W1963764148 hasRelatedWork W2042277157 @default.
- W1963764148 hasRelatedWork W2042743051 @default.
- W1963764148 hasRelatedWork W2065915398 @default.
- W1963764148 hasRelatedWork W2086026328 @default.
- W1963764148 hasRelatedWork W2114284755 @default.
- W1963764148 hasVolume "296" @default.
- W1963764148 isParatext "false" @default.
- W1963764148 isRetracted "false" @default.