Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963764211> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W1963764211 endingPage "181" @default.
- W1963764211 startingPage "177" @default.
- W1963764211 abstract "We describe a method by which complex protein mixtures are fractionated by standard one-dimensional Na-DodSO4/polyacrylamide gel electrophoresis or O'Farrell two-dimensional gel electrophoresis and then are efficiently and rapidly transferred electrophoretically to diazobenzyloxymethyl- or diazophenylthioether-paper and analyzed by immunoautoradiography. The method is illustrated with protein extracts of human KB cells infected with adenovirus type 2. Proteins were transferred from gels without decrease in resolution and with an increase in the sensitivity of detection by autoradiography when [35S]-methionine-labeled proteins were used. When unlabeled proteins were transferred, low levels of virus encoded proteins could be detected by sequential treatment of diazobenzyloxymethyl-paper with anti-adenovirus type 2 virion or anti-73,000 DNA binding protein and 125I-labeled Staphyloccus aureus protein A. Covalently bound viral proteins retained immunologic reactivity after dissociation of the protein A and antibody, By one-dimensional gel transfer/immunoautoradiography, seven virion proteins were detected as prominent bands and several others as weaker bands. By two-dimensional gel transfer/immunoautoradiography, several additional viral proteins were detected. By use of anti-DNA binding protein serum, the Mr 73,000 protein and Mr 41,000-48,000 subspecies were detected. A protein present at a concentration of approximately 1 part in 100,000 of the total protein can be identified in cell extracts. This method may be applicable to various biological problems requiring resolution and detection of small amounts of specific proteins that can be recognized immunologically or that can be detected by binding to specific radiolabeled DNA or RNA sequences or hormones." @default.
- W1963764211 created "2016-06-24" @default.
- W1963764211 creator A5030738643 @default.
- W1963764211 creator A5044303453 @default.
- W1963764211 creator A5055871307 @default.
- W1963764211 date "1981-01-01" @default.
- W1963764211 modified "2023-10-17" @default.
- W1963764211 title "Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins." @default.
- W1963764211 cites W1036870626 @default.
- W1963764211 cites W1564253751 @default.
- W1963764211 cites W1588885776 @default.
- W1963764211 cites W1606423922 @default.
- W1963764211 cites W1775749144 @default.
- W1963764211 cites W1981815989 @default.
- W1963764211 cites W2008148453 @default.
- W1963764211 cites W2015226054 @default.
- W1963764211 cites W2016613744 @default.
- W1963764211 cites W2018289835 @default.
- W1963764211 cites W2038904742 @default.
- W1963764211 cites W2068593367 @default.
- W1963764211 cites W2086195230 @default.
- W1963764211 cites W2101108802 @default.
- W1963764211 cites W2220782623 @default.
- W1963764211 doi "https://doi.org/10.1073/pnas.78.1.177" @default.
- W1963764211 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/319014" @default.
- W1963764211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7017709" @default.
- W1963764211 hasPublicationYear "1981" @default.
- W1963764211 type Work @default.
- W1963764211 sameAs 1963764211 @default.
- W1963764211 citedByCount "111" @default.
- W1963764211 countsByYear W19637642112022 @default.
- W1963764211 crossrefType "journal-article" @default.
- W1963764211 hasAuthorship W1963764211A5030738643 @default.
- W1963764211 hasAuthorship W1963764211A5044303453 @default.
- W1963764211 hasAuthorship W1963764211A5055871307 @default.
- W1963764211 hasBestOaLocation W19637642111 @default.
- W1963764211 hasConcept C101660505 @default.
- W1963764211 hasConcept C104317684 @default.
- W1963764211 hasConcept C116084860 @default.
- W1963764211 hasConcept C153911025 @default.
- W1963764211 hasConcept C181199279 @default.
- W1963764211 hasConcept C185592680 @default.
- W1963764211 hasConcept C552990157 @default.
- W1963764211 hasConcept C55493867 @default.
- W1963764211 hasConcept C86339819 @default.
- W1963764211 hasConcept C86803240 @default.
- W1963764211 hasConcept C94966510 @default.
- W1963764211 hasConceptScore W1963764211C101660505 @default.
- W1963764211 hasConceptScore W1963764211C104317684 @default.
- W1963764211 hasConceptScore W1963764211C116084860 @default.
- W1963764211 hasConceptScore W1963764211C153911025 @default.
- W1963764211 hasConceptScore W1963764211C181199279 @default.
- W1963764211 hasConceptScore W1963764211C185592680 @default.
- W1963764211 hasConceptScore W1963764211C552990157 @default.
- W1963764211 hasConceptScore W1963764211C55493867 @default.
- W1963764211 hasConceptScore W1963764211C86339819 @default.
- W1963764211 hasConceptScore W1963764211C86803240 @default.
- W1963764211 hasConceptScore W1963764211C94966510 @default.
- W1963764211 hasIssue "1" @default.
- W1963764211 hasLocation W19637642111 @default.
- W1963764211 hasLocation W19637642112 @default.
- W1963764211 hasLocation W19637642113 @default.
- W1963764211 hasLocation W19637642114 @default.
- W1963764211 hasOpenAccess W1963764211 @default.
- W1963764211 hasPrimaryLocation W19637642111 @default.
- W1963764211 hasRelatedWork W2009885565 @default.
- W1963764211 hasRelatedWork W2018781929 @default.
- W1963764211 hasRelatedWork W2049881746 @default.
- W1963764211 hasRelatedWork W2054534600 @default.
- W1963764211 hasRelatedWork W2065220711 @default.
- W1963764211 hasRelatedWork W2093585084 @default.
- W1963764211 hasRelatedWork W2134359888 @default.
- W1963764211 hasRelatedWork W2774274025 @default.
- W1963764211 hasRelatedWork W3178597674 @default.
- W1963764211 hasRelatedWork W388293201 @default.
- W1963764211 hasVolume "78" @default.
- W1963764211 isParatext "false" @default.
- W1963764211 isRetracted "false" @default.
- W1963764211 magId "1963764211" @default.
- W1963764211 workType "article" @default.