Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963769713> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1963769713 endingPage "586" @default.
- W1963769713 startingPage "582" @default.
- W1963769713 abstract "Two single-copy DNA segments of 6 kilobases (kb) and 2.3 kb were labeled with biotin-labeled dUTP (Bio11-dUTP) and hybridized to human chromosomes. These probes were detected by immunofluorescence and directly mapped on chromosomes by using classical fluorescence microscopy and a microchannel-plate-intensified video camera. By a subsequent R-banding, the 6-kb and 2.3-kb fragments were precisely localized to the 18p11.3 band and to the 22q11.2 band, respectively, in agreement with previous results obtained with radioactive probes. The adaptation of fluorescence intensification and digital image processing (frame integration to enhance signal-to-noise ratio and linear contrast stretching) to microscopy makes it possible to detect very weak fluorescent spots on chromosomes. This system allows a high spatial resolution (less than 0.6 micron), even at very low fluorescence levels. The efficiency and the specificity of the hybridization and detection methodology give a direct and precise localization of the short single-copy sequences on human chromosomes." @default.
- W1963769713 created "2016-06-24" @default.
- W1963769713 creator A5046624926 @default.
- W1963769713 creator A5066891830 @default.
- W1963769713 creator A5076932188 @default.
- W1963769713 creator A5084648075 @default.
- W1963769713 date "1989-01-01" @default.
- W1963769713 modified "2023-10-11" @default.
- W1963769713 title "Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy." @default.
- W1963769713 cites W1984365283 @default.
- W1963769713 cites W1989277922 @default.
- W1963769713 cites W1998649268 @default.
- W1963769713 cites W2000471657 @default.
- W1963769713 cites W2006984368 @default.
- W1963769713 cites W2014038908 @default.
- W1963769713 cites W2018133154 @default.
- W1963769713 cites W2028409921 @default.
- W1963769713 cites W2031643259 @default.
- W1963769713 cites W2032826590 @default.
- W1963769713 cites W2056227286 @default.
- W1963769713 cites W2060183661 @default.
- W1963769713 cites W2062715663 @default.
- W1963769713 cites W2068551399 @default.
- W1963769713 cites W2069644796 @default.
- W1963769713 cites W2079789901 @default.
- W1963769713 cites W2089679570 @default.
- W1963769713 cites W2099489108 @default.
- W1963769713 cites W2120678660 @default.
- W1963769713 doi "https://doi.org/10.1073/pnas.86.2.582" @default.
- W1963769713 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/286516" @default.
- W1963769713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2643118" @default.
- W1963769713 hasPublicationYear "1989" @default.
- W1963769713 type Work @default.
- W1963769713 sameAs 1963769713 @default.
- W1963769713 citedByCount "118" @default.
- W1963769713 countsByYear W19637697132015 @default.
- W1963769713 countsByYear W19637697132016 @default.
- W1963769713 countsByYear W19637697132017 @default.
- W1963769713 countsByYear W19637697132018 @default.
- W1963769713 countsByYear W19637697132019 @default.
- W1963769713 crossrefType "journal-article" @default.
- W1963769713 hasAuthorship W1963769713A5046624926 @default.
- W1963769713 hasAuthorship W1963769713A5066891830 @default.
- W1963769713 hasAuthorship W1963769713A5076932188 @default.
- W1963769713 hasAuthorship W1963769713A5084648075 @default.
- W1963769713 hasBestOaLocation W19637697131 @default.
- W1963769713 hasConcept C104317684 @default.
- W1963769713 hasConcept C120665830 @default.
- W1963769713 hasConcept C121332964 @default.
- W1963769713 hasConcept C130197536 @default.
- W1963769713 hasConcept C137390916 @default.
- W1963769713 hasConcept C147080431 @default.
- W1963769713 hasConcept C153911025 @default.
- W1963769713 hasConcept C169274487 @default.
- W1963769713 hasConcept C204628709 @default.
- W1963769713 hasConcept C2777542201 @default.
- W1963769713 hasConcept C30481170 @default.
- W1963769713 hasConcept C54355233 @default.
- W1963769713 hasConcept C552990157 @default.
- W1963769713 hasConcept C86803240 @default.
- W1963769713 hasConcept C91881484 @default.
- W1963769713 hasConceptScore W1963769713C104317684 @default.
- W1963769713 hasConceptScore W1963769713C120665830 @default.
- W1963769713 hasConceptScore W1963769713C121332964 @default.
- W1963769713 hasConceptScore W1963769713C130197536 @default.
- W1963769713 hasConceptScore W1963769713C137390916 @default.
- W1963769713 hasConceptScore W1963769713C147080431 @default.
- W1963769713 hasConceptScore W1963769713C153911025 @default.
- W1963769713 hasConceptScore W1963769713C169274487 @default.
- W1963769713 hasConceptScore W1963769713C204628709 @default.
- W1963769713 hasConceptScore W1963769713C2777542201 @default.
- W1963769713 hasConceptScore W1963769713C30481170 @default.
- W1963769713 hasConceptScore W1963769713C54355233 @default.
- W1963769713 hasConceptScore W1963769713C552990157 @default.
- W1963769713 hasConceptScore W1963769713C86803240 @default.
- W1963769713 hasConceptScore W1963769713C91881484 @default.
- W1963769713 hasIssue "2" @default.
- W1963769713 hasLocation W19637697131 @default.
- W1963769713 hasLocation W19637697132 @default.
- W1963769713 hasLocation W19637697133 @default.
- W1963769713 hasLocation W19637697134 @default.
- W1963769713 hasOpenAccess W1963769713 @default.
- W1963769713 hasPrimaryLocation W19637697131 @default.
- W1963769713 hasRelatedWork W1888024323 @default.
- W1963769713 hasRelatedWork W1963769713 @default.
- W1963769713 hasRelatedWork W2002473487 @default.
- W1963769713 hasRelatedWork W2011681232 @default.
- W1963769713 hasRelatedWork W2017404302 @default.
- W1963769713 hasRelatedWork W2049782758 @default.
- W1963769713 hasRelatedWork W2085759214 @default.
- W1963769713 hasRelatedWork W2106876212 @default.
- W1963769713 hasRelatedWork W2421437423 @default.
- W1963769713 hasRelatedWork W996471137 @default.
- W1963769713 hasVolume "86" @default.
- W1963769713 isParatext "false" @default.
- W1963769713 isRetracted "false" @default.
- W1963769713 magId "1963769713" @default.
- W1963769713 workType "article" @default.