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- W1963771729 abstract "Abstract Endothelial progenitor cells (EPCs) may have great potential for use as a cellular therapy for promoting vascular repair of ischaemic tissues, such as complex wounds. The term EPC, however, has been used to describe a diverse variety of cells. This ambiguity has led to conflicting results when comparing results of EPC studies from different research laboratories. Recently, there is consensus that when selective in-vitro cell culture techniques are used, two distinct EPC subpopulations are generated—namely, myeloid angiogenic cells (MACs) and outgrowth endothelial cells (OECs). This study provides a full phenotypic and functional characterisation of MACs and OECs and compares their potential therapeutic role in wound healing. EPC subtypes were isolated from human peripheral blood and umbilical cord blood. MACs appeared early in culture (∼7 days) as elongated cells with multiple fine dendritic processes and low proliferative potential. OEC colonies appeared later in culture (3–5 weeks), and formed a cobblestone-shaped monolayer. Genome-wide transcriptomics demonstrated that MACs and OECs belong to the haemopoietic and endothelial lineages, respectively. MACs cultured in vitro were further analysed, and were found to be phenotypically very similar to M2 alternative activated macrophages. Analysis of functional characteristics showed that MACs have low proliferative potential. However, OECs possess high proliferative potential, enabling them to be single-cell cloned, with an average 60 population doublings in 80 days for cord-derived OECs. Using in-vitro angiogenesis assays, OECs demonstrated de-novo tubulogenesis capacity, and were capable of interacting with mature endothelial cells through adherens and tight junctions. MACs did not form tubes and did not differentiate into endothelial cells, but significantly promoted tubulogenesis of mature endothelial cells by releasing paracrine factors such as interleukin 8 and monocyte chemoattractant protein 1. Funding Northern Ireland Medical and Dental Training Agency." @default.
- W1963771729 created "2016-06-24" @default.
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- W1963771729 date "2013-02-01" @default.
- W1963771729 modified "2023-09-26" @default.
- W1963771729 title "Characterisation and therapeutic potential of endothelial progenitor cells" @default.
- W1963771729 doi "https://doi.org/10.1016/s0140-6736(13)60513-9" @default.
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