FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1963843872> ?p ?o ?g. }

• W1963843872 endingPage "377" @default.
• W1963843872 startingPage "366" @default.
• W1963843872 abstract "Background aims Despite the availability of modern antibiotics/antimycotics and cytokine support, neutropenic infection accounts for the majority of chemotherapy-associated deaths. While transfusion support with donor neutrophils is possible, cost and complicated logistics make such an option unrealistic on a routine basis. A manufactured neutrophil product could enable routine prophylactic administration of neutrophils, preventing the onset of neutropenia and substantially reducing the risk of infection. We examined the use of pre-culture strategies and various cytokine/modulator combinations to improve neutrophil expansion from umbilical cord blood (UCB) hematopoietic stem and progenitor cells (HPC). Methods Enriched UCB HPC were cultured using either two-phase pre-culture strategies or a single phase using various cytokine/modulator combinations. Outcome was assessed with respect to numerical expansion, cell morphology, granulation and respiratory burst activity. Results Pre-culture in the absence of strong differentiation signals (e.g. granulocyte colony-stimulating factor; G-CSF) failed to provide any improvement to final neutrophil yields. Similarly, removal of differentiating cells during pre-culture failed to improve neutrophil yields to an appreciable extent. Of the cytokine/modulator combinations, the addition of granulocyte–macrophage (GM)-colony-stimulating factor (CSF) alone gave the greatest increase. In order to avoid production of monocytes, it was necessary to remove GM-CSF on day 5. Using this strategy, neutrophil expansion improved 2.7-fold. Conclusions Although all cytokines and culture strategies employed have been reported previously to enhance HPC expansion, we found that the addition of GM-CSF alone was sufficient to improve total cell yields maximally. The need to remove GM-CSF on day 5 to avoid monocyte differentiation highlights the context and time-dependent complexity of exogenous signaling in hematopoietic cell differentiation and growth." @default.
• W1963843872 created "2016-06-24" @default.
• W1963843872 creator A5010808182 @default.
• W1963843872 creator A5043169076 @default.
• W1963843872 creator A5061172047 @default.
• W1963843872 date "2011-03-01" @default.
• W1963843872 modified "2023-09-26" @default.
• W1963843872 title "Short-term exposure of umbilical cord blood CD34+ cells to granulocyte–macrophage colony-stimulating factor early in culture improves ex vivo expansion of neutrophils" @default.
• W1963843872 cites W1484778901 @default.
• W1963843872 cites W1544153210 @default.
• W1963843872 cites W155851692 @default.
• W1963843872 cites W1583818589 @default.
• W1963843872 cites W1586732863 @default.
• W1963843872 cites W1593763878 @default.
• W1963843872 cites W1684886834 @default.
• W1963843872 cites W1714982624 @default.
• W1963843872 cites W1971018369 @default.
• W1963843872 cites W1972041464 @default.
• W1963843872 cites W1976471114 @default.
• W1963843872 cites W1977253353 @default.
• W1963843872 cites W1977457971 @default.
• W1963843872 cites W1978064491 @default.
• W1963843872 cites W1979939650 @default.
• W1963843872 cites W1980024272 @default.
• W1963843872 cites W1980318916 @default.
• W1963843872 cites W1987858730 @default.
• W1963843872 cites W1988576340 @default.
• W1963843872 cites W1990792474 @default.
• W1963843872 cites W1993120779 @default.
• W1963843872 cites W1995365460 @default.
• W1963843872 cites W1995440539 @default.
• W1963843872 cites W1999507513 @default.
• W1963843872 cites W2000821014 @default.
• W1963843872 cites W2007542340 @default.
• W1963843872 cites W2009233255 @default.
• W1963843872 cites W2013118789 @default.
• W1963843872 cites W2024837696 @default.
• W1963843872 cites W2028860972 @default.
• W1963843872 cites W2034743279 @default.
• W1963843872 cites W2035301585 @default.
• W1963843872 cites W2040066514 @default.
• W1963843872 cites W2042965746 @default.
• W1963843872 cites W2044757085 @default.
• W1963843872 cites W2069078768 @default.
• W1963843872 cites W2077310831 @default.
• W1963843872 cites W2083331687 @default.
• W1963843872 cites W2100152496 @default.
• W1963843872 cites W2100476572 @default.
• W1963843872 cites W2110956170 @default.
• W1963843872 cites W2122086247 @default.
• W1963843872 cites W2128492229 @default.
• W1963843872 cites W2129321368 @default.
• W1963843872 cites W2130785007 @default.
• W1963843872 cites W2131994776 @default.
• W1963843872 cites W2161290044 @default.
• W1963843872 cites W2164713357 @default.
• W1963843872 cites W2175214965 @default.
• W1963843872 cites W2187355871 @default.
• W1963843872 cites W2197012898 @default.
• W1963843872 cites W2208532882 @default.
• W1963843872 cites W2267126247 @default.
• W1963843872 cites W2272909078 @default.
• W1963843872 cites W2278365184 @default.
• W1963843872 cites W2278505951 @default.
• W1963843872 cites W2281552814 @default.
• W1963843872 cites W228431643 @default.
• W1963843872 cites W2396977997 @default.
• W1963843872 cites W2431051467 @default.
• W1963843872 cites W299764968 @default.
• W1963843872 doi "https://doi.org/10.3109/14653249.2010.518610" @default.
• W1963843872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20860426" @default.
• W1963843872 hasPublicationYear "2011" @default.
• W1963843872 type Work @default.
• W1963843872 sameAs 1963843872 @default.
• W1963843872 citedByCount "12" @default.
• W1963843872 countsByYear W19638438722012 @default.
• W1963843872 countsByYear W19638438722013 @default.
• W1963843872 countsByYear W19638438722014 @default.
• W1963843872 countsByYear W19638438722016 @default.
• W1963843872 countsByYear W19638438722017 @default.
• W1963843872 countsByYear W19638438722019 @default.
• W1963843872 countsByYear W19638438722020 @default.
• W1963843872 crossrefType "journal-article" @default.
• W1963843872 hasAuthorship W1963843872A5010808182 @default.
• W1963843872 hasAuthorship W1963843872A5043169076 @default.
• W1963843872 hasAuthorship W1963843872A5061172047 @default.
• W1963843872 hasConcept C10205521 @default.
• W1963843872 hasConcept C109159458 @default.
• W1963843872 hasConcept C126322002 @default.
• W1963843872 hasConcept C150903083 @default.
• W1963843872 hasConcept C201750760 @default.
• W1963843872 hasConcept C203014093 @default.
• W1963843872 hasConcept C207001950 @default.
• W1963843872 hasConcept C26291073 @default.
• W1963843872 hasConcept C2776694085 @default.
• W1963843872 hasConcept C2777063308 @default.
• W1963843872 hasConcept C2777478134 @default.
• W1963843872 hasConcept C2777767877 @default.

• next