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• W1963902148 abstract "A ‘phase-shift’ translation fusion vector was constructed in which mutually compatible restriction sites BamHI, BelI and BglII are positioned in such a manner that the cut point is in a different reading frame, immediately following the ATG start codon and ribosome-binding site of the λ cro gene. The λ cro gene is expressed from promoter pR and controlled by a thermosensitive (cI857) λ repressor. The usefulness of the expression vector was demonstrated using a galϰ gene lacking the ATG start codon and fusing this to the pR promoter and ATG start codon of the λ cro gene, resulting in cI857-regulated expression of galactokinase. The vector is of general use for foreign gene expression in Escherichia coli when the target gene has a compatible cohesive end (5′-GATC-3′) at the N terminus (provided, for example, by a BamHI linker). The A λ cI857-pR-cro-galK cassette was cloned into pJRD215, a wide-host-range plasmid and transferred by conjugation to a variety of Gram-negative bacteria. In all cases, thermosensitive regulation of galactokinase could be demonstrated, though the levels of induction varied considerably. These results show that the powerful λ pR promoter and the efficient A repressor can be used to regulate expression of foreign genes in Gram-negative organisms other than E. coli." @default.
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• W1963902148 date "1987-01-01" @default.
• W1963902148 modified "2023-09-25" @default.
• W1963902148 title "A ‘phase-shift’ fusion system for the regulation of foreign gene expression by lambda represser in Gram-negative bacteria" @default.
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• W1963902148 doi "https://doi.org/10.1016/0378-1119(87)90231-9" @default.
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