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- W1963958192 abstract "We report herein a new method for the aminoacylation of tRNA, using a resin-immobilized ribozyme and the cyanomethyl ester (CME) of an amino acid substrate. The oxidized form of the ribozyme was immobilized on a hydrazine resin via covalent linkage. We performed aminoacylation of tRNAs using this ribozyme-resin to isolate aminoacyl-tRNAs. The column was recycled up to 5 times without significant activity loss. Thus, our ribozyme-based aminoacylation system has significant potential to be a powerful and practical technique for supplying various nonnatural aminoacyl-tRNAs for a highly efficient in vitro translation system." @default.
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- W1963958192 date "2002-05-22" @default.
- W1963958192 modified "2023-10-12" @default.
- W1963958192 title "Aminoacyl-tRNA Synthesis by a Resin-Immobilized Ribozyme" @default.
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- W1963958192 doi "https://doi.org/10.1021/ja025872a" @default.
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