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- W1963981598 abstract "A new CE-MS method with enzymatic reaction inside the capillarywas developed for the study of drug metabolism by cytochromesP450. This automated method, based on the transverse diffusionof laminar flow profiles methodology, is comprised of theinjection of substrates and enzyme, their mixing, incubationand separation of the reaction products, all performed by CE,and their detection, identification and quantification by MS.The developed and validated method was finally used to conducta kinetic study of cytochrome P450 isoform 2C9 or human livermicrosomes with diclofenac in order to demonstrate itspractical functionality. All the estimated kinetic values –apparent Michaelis constants and apparent maximum reactionvelocities were in agreement with literature data obtainedusing other techniques. In addition, the consumption ofreactants was in the tens of nL per analysis. The method’susability was further demonstrated on tolbutamide, the otherprobe substrate of cytochrome P450 isoform 2C9. As a result,the method is conceptually applicable for the screening of anyother cytochromes P450 isoform and its substrates andinhibitors after adapting the incubation and separationconditions." @default.
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- W1963981598 date "2015-01-29" @default.
- W1963981598 modified "2023-10-16" @default.
- W1963981598 title "Combination of on-line capillary electrophoretic assay withmass spectrometry detection for the study of drug metabolism bycytochromes P450" @default.
- W1963981598 hasPublicationYear "2015" @default.
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