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• W1964009242 abstract "Immunoglobulin expression was studied by direct immunofluorescence and by biosynthesis experiments in two human cell lines Raji and T 5.1. The basic phenotype of these cells was close to that of pre-B cells: large cells with intracytoplasmic IgM with a predominance of mu chains over light chains and no detectable surface immunoglobulins. The apparent molecular weight of heavy and light chains was abnormally large. Immunoglobulins were secreted at a low rate as pentameric IgM in the T 5.1 line and as subunits and free light chains in the Raji line. Spontaneous variations of this phenotype were observed: the cultured cells acquired mu and lambda chains, then additionally delta chains while they progressively lost detectable cytoplasmic mu chains, thus leading to a mature B cell phenotype. Subsequently, the cells had no detectable surface and cytoplasmic immunoglobulins and then they displayed a pre-B cell phenotype again. Attempts to induce further maturation using various potential inducers were unsuccessful." @default.
• W1964009242 created "2016-06-24" @default.
• W1964009242 creator A5003808490 @default.
• W1964009242 creator A5077298115 @default.
• W1964009242 date "1981-04-01" @default.
• W1964009242 modified "2023-09-27" @default.
• W1964009242 title "Immunoglobulin Expression in Human Lymphoblastoid Cell Lines with Early B Cell Features" @default.
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• W1964009242 doi "https://doi.org/10.1111/j.1365-3083.1981.tb00139.x" @default.
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