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- W1964091685 abstract "An in-house assembled electrochromatograph was used for temperature-controlled non-aqueous electrochromatography. Reversed-phase separations of retinyl esters were performed on continuous bed columns. The continuous bed columns were prepared by sol-gel bonding of C30 material in 180 μm internal diameter fused silica capillaries. The mobile phase consisted of 2.5 mM lithium acetate in N,N-dimethylformamide-acetonitrile-methanol (2 + 7 + 1, v/v). The use of temperature programming increased the resolution of earlier-eluting compounds as well as improved the peak shape and decreased the retention time of later-eluting compounds. Separations of retinyl esters (all-trans-retinyl acetate, palmitate, heptadecanoate, stearate, oleate, and linoleate) were completed in 12 minutes. The within-day and between-day variations of retention times of all-trans-retinyl palmitate were <0.6% (n = 6) relative standard deviation (RSD) and 2.3% (n = 3) RSD, respectively. Further, the within-day and between-day variations of peak areas were < 2.7% (n = 6) RSD, and 3.4% (n = 3) RSD, respectively. The column examined was stable for more than two weeks of continuous injections of standard solutions and real samples. Lipid extracts of bearded seal (Erignathus barbatus) liver were analyzed." @default.
- W1964091685 created "2016-06-24" @default.
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- W1964091685 date "2001-06-01" @default.
- W1964091685 modified "2023-10-04" @default.
- W1964091685 title "Temperature-programmed non-aqueous electrochromatographic separation of retinyl esters" @default.
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- W1964091685 doi "https://doi.org/10.1002/1615-9314(20010601)24:6<435::aid-jssc435>3.0.co;2-5" @default.
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