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- W1964163399 abstract "Abstract Previous studies have demonstrated an elevated Na + -H + exchanger activity in various cell types from patients with essential hypertension. The phenotype of an increased maximal transport capacity is preserved in Epstein-Barr virus immortalized lymphoblasts from hypertensive patients. The mechanisms underlying this abnormality are unclear. In this study, we used lymphoblasts from hypertensive patients and normotensive control subjects with and without a family history of hypertension to determine (1) Na + -H + exchanger activity using fluorometry with the pH indicator 2′,7′-bis(carboxyethyl)-5(6)-carboxyfluorescein, (2) Na + -H + exchanger isoform 1 abundance with specific polyclonal antibodies, and (3) Na + -H + exchanger phosphorylation by immunoprecipitation of the 32 P-labeled transporter. Na + -H + exchanger activity (in millimoles per liter per minute) measured when pH i was clamped at 6.0 was significantly higher in cells from hypertensive patients (18.8±0.6, P <.001) and those subjects with a family history of hypertension (16.4±0.6, P <.001) compared with normotensive control subjects (12.9±0.6). Exchanger abundance was identical in all three groups of subjects, indicating that increased activity in the hypertensive group was due to an elevated turnover number of the exchanger. Na + -H + exchanger phosphorylation in quiescent cells was significantly elevated in cells from hypertensive patients (1.58±0.16, P <.001) compared with control subjects (1.00±0.07), and cells from normotensive subjects with a hypertensive family history showed intermediate values (1.23±0.14). Identical changes in Na + -H + exchanger function and phosphorylation have been demonstrated in vascular smooth muscle cells from spontaneously hypertensive rats. Our findings suggest that the elevated Na + -H + exchanger activity in cells from human hypertensive patients is not associated with an increased exchanger abundance but may be related to increased exchanger phosphorylation." @default.
- W1964163399 created "2016-06-24" @default.
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- W1964163399 date "1995-05-01" @default.
- W1964163399 modified "2023-09-23" @default.
- W1964163399 title "Na + -H + Antiporter Phenotype, Abundance, and Phosphorylation of Immortalized Lymphoblasts From Humans With Hypertension" @default.
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- W1964163399 doi "https://doi.org/10.1161/01.hyp.25.5.971" @default.
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