FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964184395> ?p ?o ?g. }

• W1964184395 endingPage "3137" @default.
• W1964184395 startingPage "3136" @default.
• W1964184395 abstract "The Tetrahymena group I intron recognizes its oligonucleotide substrate in a two-step process. First, a substrate recognition duplex, called the P1 duplex, is formed. The P1 duplex then docks into the prefolded ribozyme core by forming tertiary contacts. P1 docking controls both the rate and the fidelity of substrate cleavage and has been extensively studied as a model for the formation of RNA tertiary structure. However, previous work has been limited to studying millisecond or slower motions. Here we investigated nanosecond P1 motions in the context of the ribozyme using site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) spectroscopy. A nitroxide spin label (R5a) was covalently attached to a specific site of the substrate oligonucleotide, the labeled substrate was bound to a prefolded ribozyme to form the P1 duplex, and X-band EPR spectroscopy was used to monitor nitroxide motions in the 0.1-50 ns regime. Using substrates that favor the docked or the undocked states, it was established that R5a was capable of reporting P1 duplex motions. Using R5a-labeled substrates it was found that the J1/2 junction connecting P1 to the ribozyme core controls nanosecond P1 mobility in the undocked state. This may account for previous observations that J1/2 mutations weaken substrate binding and give rise to cryptic cleavage. This study establishes the use of SDSL to probe nanosecond dynamic behaviors of individual helices within large RNA and RNA/protein complexes. This approach may help in understanding the relationship between RNA structure, dynamics, and function." @default.
• W1964184395 created "2016-06-24" @default.
• W1964184395 creator A5056575484 @default.
• W1964184395 creator A5066711431 @default.
• W1964184395 creator A5073038176 @default.
• W1964184395 creator A5076625801 @default.
• W1964184395 date "2009-02-16" @default.
• W1964184395 modified "2023-09-23" @default.
• W1964184395 title "Motions of the Substrate Recognition Duplex in a Group I Intron Assessed by Site-Directed Spin Labeling" @default.
• W1964184395 cites W1760751973 @default.
• W1964184395 cites W1966307609 @default.
• W1964184395 cites W1968282235 @default.
• W1964184395 cites W1978921989 @default.
• W1964184395 cites W1988744922 @default.
• W1964184395 cites W1994519311 @default.
• W1964184395 cites W2006064177 @default.
• W1964184395 cites W2007552023 @default.
• W1964184395 cites W2038492165 @default.
• W1964184395 cites W2058637503 @default.
• W1964184395 cites W2068146975 @default.
• W1964184395 cites W2069034875 @default.
• W1964184395 cites W2076499829 @default.
• W1964184395 cites W2093003807 @default.
• W1964184395 cites W2128610236 @default.
• W1964184395 cites W2128677332 @default.
• W1964184395 cites W2133296358 @default.
• W1964184395 cites W2141885436 @default.
• W1964184395 cites W2158875436 @default.
• W1964184395 cites W2952587452 @default.
• W1964184395 doi "https://doi.org/10.1021/ja808217s" @default.
• W1964184395 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2788000" @default.
• W1964184395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19220053" @default.
• W1964184395 hasPublicationYear "2009" @default.
• W1964184395 type Work @default.
• W1964184395 sameAs 1964184395 @default.
• W1964184395 citedByCount "42" @default.
• W1964184395 countsByYear W19641843952012 @default.
• W1964184395 countsByYear W19641843952013 @default.
• W1964184395 countsByYear W19641843952014 @default.
• W1964184395 countsByYear W19641843952015 @default.
• W1964184395 countsByYear W19641843952016 @default.
• W1964184395 countsByYear W19641843952017 @default.
• W1964184395 countsByYear W19641843952018 @default.
• W1964184395 countsByYear W19641843952020 @default.
• W1964184395 countsByYear W19641843952021 @default.
• W1964184395 countsByYear W19641843952022 @default.
• W1964184395 crossrefType "journal-article" @default.
• W1964184395 hasAuthorship W1964184395A5056575484 @default.
• W1964184395 hasAuthorship W1964184395A5066711431 @default.
• W1964184395 hasAuthorship W1964184395A5073038176 @default.
• W1964184395 hasAuthorship W1964184395A5076625801 @default.
• W1964184395 hasBestOaLocation W19641843952 @default.
• W1964184395 hasConcept C104317684 @default.
• W1964184395 hasConcept C107745601 @default.
• W1964184395 hasConcept C121332964 @default.
• W1964184395 hasConcept C12554922 @default.
• W1964184395 hasConcept C126142528 @default.
• W1964184395 hasConcept C129312508 @default.
• W1964184395 hasConcept C177462420 @default.
• W1964184395 hasConcept C185592680 @default.
• W1964184395 hasConcept C187961010 @default.
• W1964184395 hasConcept C41625074 @default.
• W1964184395 hasConcept C46141821 @default.
• W1964184395 hasConcept C552990157 @default.
• W1964184395 hasConcept C55493867 @default.
• W1964184395 hasConcept C67705224 @default.
• W1964184395 hasConcept C71240020 @default.
• W1964184395 hasConcept C76346623 @default.
• W1964184395 hasConcept C8010536 @default.
• W1964184395 hasConcept C86803240 @default.
• W1964184395 hasConcept C99611785 @default.
• W1964184395 hasConceptScore W1964184395C104317684 @default.
• W1964184395 hasConceptScore W1964184395C107745601 @default.
• W1964184395 hasConceptScore W1964184395C121332964 @default.
• W1964184395 hasConceptScore W1964184395C12554922 @default.
• W1964184395 hasConceptScore W1964184395C126142528 @default.
• W1964184395 hasConceptScore W1964184395C129312508 @default.
• W1964184395 hasConceptScore W1964184395C177462420 @default.
• W1964184395 hasConceptScore W1964184395C185592680 @default.
• W1964184395 hasConceptScore W1964184395C187961010 @default.
• W1964184395 hasConceptScore W1964184395C41625074 @default.
• W1964184395 hasConceptScore W1964184395C46141821 @default.
• W1964184395 hasConceptScore W1964184395C552990157 @default.
• W1964184395 hasConceptScore W1964184395C55493867 @default.
• W1964184395 hasConceptScore W1964184395C67705224 @default.
• W1964184395 hasConceptScore W1964184395C71240020 @default.
• W1964184395 hasConceptScore W1964184395C76346623 @default.
• W1964184395 hasConceptScore W1964184395C8010536 @default.
• W1964184395 hasConceptScore W1964184395C86803240 @default.
• W1964184395 hasConceptScore W1964184395C99611785 @default.
• W1964184395 hasIssue "9" @default.
• W1964184395 hasLocation W19641843951 @default.
• W1964184395 hasLocation W19641843952 @default.
• W1964184395 hasLocation W19641843953 @default.
• W1964184395 hasLocation W19641843954 @default.
• W1964184395 hasOpenAccess W1964184395 @default.
• W1964184395 hasPrimaryLocation W19641843951 @default.
• W1964184395 hasRelatedWork W1976796482 @default.

• next