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• W1964211232 abstract "Troponin T (TnT) is the tropomyosin (Tm) binding subunit of the troponin complex that mediates the Ca2+ regulation of actomyosin interaction in striated muscles. Troponin T isoform diversity is marked by a developmentally regulated acidic to basic switch that may modulate muscle contractility. We previously reported that transgenic expression of fast skeletal muscle TnT altered the cooperativity of cardiac muscle. In the present study, we have demonstrated that the binding of acidic TnT to troponin I is weaker than that of basic TnT. However, affinity chromatography experiments showed that Tm bound to acidic TnT with a greater affinity than to basic TnT, consistent with the significantly higher maximal binding of acidic TnT to Tm in solid phase binding assays. Competition and co-immunoprecipitation experiments demonstrated that the binding of TnT to Tm was cooperative in the absence of F-actin. The cooperativity between TnT molecules for Tm binding can be initiated by the conserved COOH-terminal T2 fragment of TnT. This indicates that the interaction of TnT with Tm induces a conformational change in Tm, promoting interaction of TnT with adjacent Tm dimers. This finding suggests a role for TnT and its acidic and basic isoforms in the cooperative release of the inhibition of striated muscle actomyosin interaction. Troponin T (TnT) is the tropomyosin (Tm) binding subunit of the troponin complex that mediates the Ca2+ regulation of actomyosin interaction in striated muscles. Troponin T isoform diversity is marked by a developmentally regulated acidic to basic switch that may modulate muscle contractility. We previously reported that transgenic expression of fast skeletal muscle TnT altered the cooperativity of cardiac muscle. In the present study, we have demonstrated that the binding of acidic TnT to troponin I is weaker than that of basic TnT. However, affinity chromatography experiments showed that Tm bound to acidic TnT with a greater affinity than to basic TnT, consistent with the significantly higher maximal binding of acidic TnT to Tm in solid phase binding assays. Competition and co-immunoprecipitation experiments demonstrated that the binding of TnT to Tm was cooperative in the absence of F-actin. The cooperativity between TnT molecules for Tm binding can be initiated by the conserved COOH-terminal T2 fragment of TnT. This indicates that the interaction of TnT with Tm induces a conformational change in Tm, promoting interaction of TnT with adjacent Tm dimers. This finding suggests a role for TnT and its acidic and basic isoforms in the cooperative release of the inhibition of striated muscle actomyosin interaction. troponin T troponin C troponin I 1,4-piperazinediethanesulfonic acid bovine serum albumin enzyme-linked immunosorbant assay tris(carboxyethyl)phosphine monoclonal antibody polyacrylamide gel electrophoresis tropomyosin chicken acidic fast skeletal muscle TnT bovine cardiac TnT Striated muscle contraction is regulated by Ca2+through the troponin-tropomyosin signaling pathway (1Leavis P.C. Gergely J. CRC Crit. Rev. Biochem. 1984; 16: 235-305Crossref PubMed Scopus (326) Google Scholar). The troponin complex, identified by Ebashi (2Ebashi S. Nature. 1963; 200: 1010Crossref PubMed Scopus (135) Google Scholar), contains three constituents (3Greaser M.L. Gergely J. J. Biol. Chem. 1971; 246: 4226-4233Abstract Full Text PDF PubMed Google Scholar): troponin T (TnT)1 (the tropomyosin (Tm) binding subunit), the inhibitory subunit troponin I (TnI), and the Ca2+-binding subunit troponin C (TnC). The binding of Ca2+ to TnC triggers a series of interactions which relieves troponin-tropomyosin inhibition of the actomyosin ATPase and allows for cross-bridge attachment and cycling (for review, see Ref. 4Tobacman L.S. Annu. Rev. Physiol. 1996; 58: 447-481Crossref PubMed Scopus (460) Google Scholar). Troponin T is at a central position in the thin filament regulatory system where it interacts with TnI (5Pearlstone J.R. Smillie L.B. Can. J. Biochem. Cell Biol. 1985; 63: 212-218Crossref PubMed Scopus (61) Google Scholar), TnC (6Pearlstone J.R. Smillie L.B. Can. J. Biochem. 1978; 56: 521-527Crossref PubMed Scopus (50) Google Scholar), Tm (7Pearlstone J.R. Smillie L.B. J. Biol. Chem. 1982; 257: 10587-10592Abstract Full Text PDF PubMed Google Scholar), and actin (8Heeley D.H. Smillie L.B. Biochemistry. 1988; 27: 8227-8232Crossref PubMed Scopus (18) Google Scholar). Three genes are responsible for the expression of TnT specific to fast skeletal, slow skeletal, and cardiac muscle fibers of vertebrates (9Cooper T.A. Ordahl C.P. J. Biol. Chem. 1985; 260: 11140-11148Abstract Full Text PDF PubMed Google Scholar, 10Breitbart R.E. Nadal-Ginard B. J. Mol. Biol. 1986; 188: 313-324Crossref PubMed Scopus (109) Google Scholar, 11Huang Q.-Q. Chen A. Jin J.-P. Gene ( Amst. ). 1999; 229: 1-10Crossref PubMed Scopus (45) Google Scholar). For each TnT gene, RNA splicing generates isoform diversity primarily through alternative exons encoding the extended NH2-terminal domain of the protein. The isoform diversity is best exemplified by fast skeletal muscle TnT wherein at least six (mammalian) and as many as thirteen (avian) exons are alternatively spliced to generate a large number of isoforms (10Breitbart R.E. Nadal-Ginard B. J. Mol. Biol. 1986; 188: 313-324Crossref PubMed Scopus (109) Google Scholar,12Smillie L.B. Golosinska K. Reinach F.C. J. Biol. Chem. 1988; 263: 18816-18820Abstract Full Text PDF PubMed Google Scholar, 13Briggs M.M. Schachat F. Dev. Biol. 1993; 158: 503-509Crossref PubMed Scopus (52) Google Scholar, 14Schachat F. Schmidt J.M. Maready M. Briggs M.M. Dev. Biol. 1995; 171: 233-239Crossref PubMed Scopus (28) Google Scholar, 15Wang J. Jin J.-P. Gene ( Amst. ). 1997; 193: 105-114Crossref PubMed Scopus (69) Google Scholar, 16Bucher E.A. Dhoot G.K. Emerson M.M. Ober M. Emerson Jr., C.P. J. Biol. Chem. 1999; 274: 17661-17670Abstract Full Text Full Text PDF PubMed Scopus (22) Google Scholar, 17Miyazaki J. Jozaki M. Nakatani N. Watanabe T. Saba R. Nakada K. Hirabayashi T. Yonemura I. J. Muscle Res. Cell Motil. 1999; 20: 655-660Crossref PubMed Scopus (15) Google Scholar). As a result of tissue-specific regulation by alternative RNA splicing, cardiac and fast skeletal muscle TnT isoform switches are observed during mammalian and avian development (9Cooper T.A. Ordahl C.P. J. Biol. Chem. 1985; 260: 11140-11148Abstract Full Text PDF PubMed Google Scholar, 13Briggs M.M. Schachat F. Dev. Biol. 1993; 158: 503-509Crossref PubMed Scopus (52) Google Scholar, 15Wang J. Jin J.-P. Gene ( Amst. ). 1997; 193: 105-114Crossref PubMed Scopus (69) Google Scholar, 18Jin J.-P. Lin J.J.-C. J. Biol. Chem. 1988; 263: 7309-7315Abstract Full Text PDF PubMed Google Scholar). This developmental expression pattern can be classified by the predicted physical properties of the TnT isoforms expressed (15Wang J. Jin J.-P. Gene ( Amst. ). 1997; 193: 105-114Crossref PubMed Scopus (69) Google Scholar). Specifically, fetal and neonatal TnT isoforms are higher M risoforms when compared with those expressed in adult striated muscles. Protein and cDNA sequencing have shown that the NH2-terminal variable region of TnT is abundant with acidic amino acid residues. Nonetheless, the NH2-terminal domains of high M r fetal and neonatal TnT isoforms have relatively acidic isoelectric points (pI) in comparison with adult TnT isoforms. The high to low M r, acidic to basic developmental TnT isoform switch is conserved among species and may reflect the role of TnT isoforms in the functional adaptation of developing striated muscles. Cardiac (18Jin J.-P. Lin J.J.-C. J. Biol. Chem. 1988; 263: 7309-7315Abstract Full Text PDF PubMed Google Scholar) and slow skeletal muscle (19Jin J.-P. Chen A Huang Q-Q Gene ( Amst. ). 1998; 214: 121-129Crossref PubMed Scopus (64) Google Scholar) TnT have more acidic NH2-terminal domains compared with those in the fast skeletal muscle TnT (15Wang J. Jin J.-P. Gene ( Amst. ). 1997; 193: 105-114Crossref PubMed Scopus (69) Google Scholar), consistent with a role for TnT isoforms in the functional diversity of muscle fiber types. The regulation of muscle regulatory protein isoforms is not restricted to TnT because TnI and Tm show muscle specific and developmental control of gene expression (20Koppe R.I. Hallauer P.L. Karpati G. Hastings K.E. J. Biol. Chem. 1989; 264: 14327-14333Abstract Full Text PDF PubMed Google Scholar, 21Saggin L. Gorza L. Ausoni S. Schiaffino S. J. Biol. Chem. 1989; 264: 16299-16302Abstract Full Text PDF PubMed Google Scholar, 22Lees-Miller J.P. Helfman D.M. Bioessays. 1991; 13: 429-437Crossref PubMed Scopus (299) Google Scholar). This suggests that expression of the muscle thin filament protein genes is coordinated. The importance of the TnT structure-function relationship to muscle contractility is further demonstrated by changes in cardiac TnT isoform expression during diabetes (23Akella A.B. Ding X.L. Cheng R. Gulati J. Circ. Res. 1995; 76: 600-606Crossref PubMed Scopus (101) Google Scholar) and heart failure (24Anderson P.A. Greig A. Mark T.A. Malouf N.N. Oakeley A.E. Ungerleider R.M. Allen P.D. Kay B.K. Circ. Res. 1995; 76: 681-686Crossref PubMed Scopus (203) Google Scholar). In addition, differences in TnT isoform expression are able to alter the sensitivity and the cooperativity of the force response to Ca2+ (25Ogut O. Granzier H. Jin J.-P. Am. J. Physiol. 1999; 276: C1162-1170Crossref PubMed Google Scholar, 26Huang Q.-Q. Brozovich F.V. Jin J.-P. J. Physiol. ( Lond. ). 1999; 520 (a): 231-242Crossref PubMed Scopus (40) Google Scholar). In this respect, TnT isoform expression may be important in the etiological or compensatory mechanism involved in compromised contractility of cardiac muscle. Therefore, understanding the interaction of acidic and basic TnT isoforms with other thin filament proteins is important in deciphering the role of TnT in Ca2+ regulation of striated muscle contraction. In the present study, we have investigated the functional properties of high M r acidic versus lowM r basic TnT isoforms. Interestingly, acidic and basic TnT show differential interactions with Tm isoforms and TnI. In addition, the binding of TnT to Tm is cooperative in the absence of F-actin, an effect that is induced by the binding of the COOH-terminal domain of TnT to Tm. Taken together, the data imply that TnT isoforms and their interactions with Tm play important roles in the physiology of muscle contraction. The anti-TnT monoclonal antibodies (mAbs) 3E4 and 6B8 (27Wang J. Jin J.-P. Biochemistry. 1998; 37: 14519-14528Crossref PubMed Scopus (75) Google Scholar) were used to specifically detect acidic and basic fast skeletal muscle TnT isoforms. The relative affinities of the mAbs to acidic and basic TnT were demonstrated by indirect enzyme-linked immunosorbant assay (ELISA). Chicken TnT was diluted to 5 μg/ml in Buffer A (10 mm PIPES, pH 7.0, 100 mm KCl, 3 mm MgCl2, 0.1 mm TCEP) and was coated overnight (100 μl/well, 4 °C) onto triplicate wells of microtiter plates (Falcon 3915). Control wells were coated with bovine serum albumin (BSA) at a concentration of 5 μg/ml. The wells were then washed once in Buffer A with 0.05% Tween 20 (Buffer T) and blocked at room temperature for 90 min using Buffer T containing 1% BSA. Following blocking, the wells were washed three times in Buffer T and incubated with serial dilutions of mAb 3E4 or 6B8 in Buffer T with 0.1% BSA for 90 min at room temperature. For antibody incubations, TCEP was omitted. The bound mAbs were detected by horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody and the H2O2 and 2,2′-azinobis-(3-ethylbenzthiazolinesulfonic acid) substrate reaction as described previously (28Ogut O. Jin J.-P. Biochemistry. 1996; 35: 16581-16590Crossref PubMed Scopus (38) Google Scholar). Troponin T and TnI were prepared from fresh chicken skeletal muscles using a protocol developed by Dr. L. B. Smillie (University of Alberta, Edmonton, Canada). As demonstrated previously (29Ogut O. Jin J.-P. J. Biol. Chem. 1998; 273: 27858-27866Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar), adult chicken breast muscle exclusively expresses high M r acidic fast skeletal muscle TnT whereas the gastrocnemius muscle exclusively expresses low M r basic fast skeletal muscle TnT. Therefore, the two muscle types were used to purify acidic and basic TnT isoforms, respectively. Following homogenization and extraction of fresh muscle with 50 mm KCl to remove soluble proteins, troponin and Tm were extracted with 0.75 mLiCl2 at pH 4.5. The supernatant was adjusted to pH 7.5 (20 min) and then lowered to pH 4.5 (20 min) to precipitate Tm. Troponin in the supernatant was then fractionated by (NH4)2SO4 at 35–65% saturation, and the precipitate was dialyzed against 0.01 N HCl until conductivity was less than 2 millisiemens/cm. Following dialysis, the pH of the solution was raised to pH 4, and the supernatant was clarified by centrifugation (the precipitate contained TnC). Solid urea and sodium acetate were added to the supernatant to 6 m and 0.01m, respectively, and the TnT/TnI mixture was fractionated by CM52-cation exchange chromatography at pH 4. The column was developed with a 0–500 mm NaCl gradient. The TnT and TnI peaks were identified by 180:1 acrylamide/bisacrylamide 14% SDS-PAGE, pooled and dialyzed against 0.01% formic acid to remove urea and salt, and lyophilized. To further purify TnT and TnI to homogeneity, the lyophilized powders were dissolved in 6 murea, 20 mm Tris-HCl, pH 8, for fractionation by DE52-anion exchange chromatography. Pure TnT and TnI eluted from the columns were dialyzed against 0.01% formic acid and lyophilized and stored at −20 °C until use. All steps were performed at 4 °C, and buffers included 0.1 mm EDTA and 0.5 mmphenylmethylsulfonyl fluoride to inhibit proteases and 5 mmβ-mercaptoethanol as a reducing agent. The preparation and purity of the chicken fast skeletal muscle TnT NH2-terminal fragment N165 (TnT-(1-165)) was previously demonstrated (28Ogut O. Jin J.-P. Biochemistry. 1996; 35: 16581-16590Crossref PubMed Scopus (38) Google Scholar). The chymotryptic T2 fragment of rabbit fast skeletal muscle TnT (30Pearlstone J.R. Smillie L.B. FEBS Lett. 1981; 128: 119-122Crossref PubMed Scopus (45) Google Scholar) was a gift from Dr. L. B. Smillie. Tropomyosins were purified according to the protocol described by Smillie (31Smillie L.B. Methods Enzymol. 1982; 85: 234-241Crossref PubMed Scopus (227) Google Scholar). A mixed population of α/β-Tm was isolated from chicken gastrocnemius muscle whereas α/α-Tm was purified from chicken breast muscle (25Ogut O. Granzier H. Jin J.-P. Am. J. Physiol. 1999; 276: C1162-1170Crossref PubMed Google Scholar). After homogenization and extraction as above, the 0.75 m LiCl2 extracts of chicken breast or gastrocnemius muscles were lowered to pH 4.5 for isoelectric precipitation of Tm. The precipitated Tm was resolubilized in 1m KCl, pH 7, for 30 min and precipitated again by decreasing the pH to 4.5. The isoelectric precipitation and resolubilization were repeated four times followed by (NH4)2SO4 fractionation to collect the 50–60% saturation pellet. The purified Tm was dialyzed extensively against double-distilled H2O and lyophilized. All steps were performed at 4 °C and included phenylmethylsulfonyl fluoride and β-mercaptoethanol as indicated above. The ratios of α- to β-Tm in the preparations were determined by densitometry of SDS-PAGE-resolved Tm isoform bands using NIH Image 1.61 software. Purity of the protein preparations was assessed by 29:1 acrylamide/bisacrylamide 12% SDS-PAGE followed by immunoblotting with TnT, TnI, or Tm antibodies to assure no cross-contamination or proteolytic degradation products in the samples. Protein concentrations were determined by absorbance measurements and SDS-PAGE analyses against controls of known concentrations. The following extinction coefficients and molecular weights were used: acidic fast skeletal muscle TnT, E 280 = 0.44 (1 mg/ml),M r = 33,000; basic fast skeletal muscle TnT,E 280 = 0.44 (1 mg/ml), M r= 29,000; chicken fast skeletal muscle TnI, E 280= 0.52 (1 mg/ml), M r = 21,000; chicken α/α- or α/β-Tm, E 280 = 0.44 (1 mg/ml),M r = 66,000 (32Lehrer S.S. Morris E.P. J. Biol. Chem. 1982; 257: 8073-8080Abstract Full Text PDF PubMed Google Scholar, 33Tobacman L.S. Lee R. J. Biol. Chem. 1987; 262: 4059-4064Abstract Full Text PDF PubMed Google Scholar). The relative binding strengths of acidic and basic TnT to α/β-Tm were determined using affinity chromatography. Purified chicken α/β-Tm was coupled to CNBr-activated Sepharose 4B gel (Amersham Pharmacia Biotech) according to the manufacturer's instructions using 11 mg of protein per 0.5 ml of gel during coupling. A 0.5-ml column was packed and equilibrated with 100 mm NaCl, 20 mm PIPES, pH 7.0, 1 mm EGTA, 0.1 mm TCEP. Acidic and basic chicken fast TnT (1.5 nmol each) were dissolved as a mixture in 10 ml of the equilibration buffer (final TnT concentration of 0.3 μm) and loaded onto the column at a flow rate of ∼0.2 ml/min. The column was washed with three volumes of the equilibration buffer and eluted by a gradient of 100–600 mm NaCl in the equilibration buffer (20-ml total volume, 0.5-ml fractions). All steps were carried out at room temperature. The fractions were resolved by 180:1 acrylamide/bisacrylamide 14% SDS-PAGE gel and silver stained to determine the high M r acidic and lowM r basic TnT peaks. For silver staining, the resolved SDS-PAGE gel was shaken for 20 min in 20 volumes of 50% methanol, 12% trichloroacetic acid, 2% CuCl2, followed by 10 min in 10% ethanol, 5% acetic acid and then 10 min in 0.01% KMnO4. The gel was then shaken in 10% ethanol, 5% acetic acid for 10 min, in 10% ethanol for 10 min, and in double-distilled H2O for 10 min and then incubated in 0.1% AgNO3 for 10 min. Following incubation in AgNO3, the gel was rinsed in double-distilled H2O for 30 s, shaken in 10% K2CO3 for 60 s, and then incubated in 0.01% formaldehyde, 2% K2CO3 until the protein bands were clearly visible. The gel was then briefly incubated in 10% methanol, 5% acetic acid and photographed. To study the high affinity interactions of TnT isoforms with TnI or Tm, we used a solid phase protein binding assay (29Ogut O. Jin J.-P. J. Biol. Chem. 1998; 273: 27858-27866Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). Chicken TnI, α/α- or α/β-Tm, or BSA were separately dissolved to 5 μg/ml in Buffer A and coated overnight (100 μl/well, 4 °C) onto triplicate wells of microtiter plates (Falcon 3915). The plates were then washed with Buffer T and blocked for 90 min at room temperature with Buffer T containing 1% BSA. Following three washes with Buffer T, the plates were incubated with serial dilutions of acidic or basic fast TnT in Buffer T with 0.1% BSA for 90 min at room temperature. The bound TnT was detected by mAb 3E4 (1:2000 dilution) using the ELISA procedure described above. The results are presented as the average ± S.D. The binding curves of TnT to Tm and TnI were well fit by single exponentials. The fits were used to determine the concentration of TnT required for 50% maximal binding. Differences in the concentration of TnT required to achieve 50% maximal binding were deemed significant ifp < 0.05 by Student's t test. The competition and cooperativity between acidic and basic TnT for Tm binding was monitored by TnT isoform-specific antibodies. We developed a solid phase ELISA method where the binding of acidic fast TnT to immobilized Tm was selectively detected by mAb 6B8 (see Fig.2 B) in the presence of basic fast TnT or bovine cardiac TnT. Briefly, following coating of Tm isoforms or BSA in Buffer A (5 μg/ml), the plates were blocked with Buffer T with 1% BSA. The immobilized Tm was incubated with TnT mixtures (in Buffer T, 0.1% BSA) wherein the concentration of acidic TnT remained constant (25 nm), but the concentration of basic or bovine cardiac TnT was titrated from 0 to 2.5 μm. Following incubation of Tm with TnT for 1.5 h, the binding of acidic TnT to Tm was determined by mAb 6B8 (1:2000 dilution). The detection of the bound antibody and color development was done as detailed above. The interaction of TnT isoforms with immobilized TnI was also analyzed using the same ELISA protocol. To determine the effect of the NH2- and COOH-terminal domains of TnT on the cooperative binding of full-length TnT to Tm, the NH2-terminal N165 fragment of chicken fast skeletal muscle TnT (Ref. 28Ogut O. Jin J.-P. Biochemistry. 1996; 35: 16581-16590Crossref PubMed Scopus (38) Google Scholar, equivalent to the chymotryptic fragment T1 (7Pearlstone J.R. Smillie L.B. J. Biol. Chem. 1982; 257: 10587-10592Abstract Full Text PDF PubMed Google Scholar)) and the COOH-terminal T2 fragment of rabbit skeletal muscle TnT were used in serial dilutions as the competing TnT species. For these experiments, mAb 3E4 (1:2000) was used to selectively detect bound full-length acidic TnT. This mAb does not cross-react to fragment N165 or the T2 fragment (27Wang J. Jin J.-P. Biochemistry. 1998; 37: 14519-14528Crossref PubMed Scopus (75) Google Scholar). The ELISA titration results are presented as the average ± S.D. The competition-cooperativity curves were fit manually. We measured the competition of TnT isoforms for Tm binding by co-immunoprecipitation. In these assays, the concentration of chicken acidic fast skeletal muscle TnT and chicken breast muscle α/α-Tm dimers were fixed at 0.1 μm and 0.05 μm, respectively. An increasing concentration of bovine cardiac TnT (0–0.1 μm) was used as a competitor of the acidic fast skeletal muscle TnT for α/α-Tm binding. The TnT/Tm mixture was incubated for 2 h at room temperature in 100 mm NaCl, 10 mm PIPES, pH 7.0, 3 mmMgCl2, 0.05% Tween 20. Following the initial incubation, anti-Tm mAb CH1 was added (∼3 μg) and was allowed to incubate for 1 h at room temperature. The antibody-bound TnT-Tm complexes were precipitated by incubation with Protein A-Sepharose beads (Amersham Pharmacia Biotech) for 35 min at room temperature. Following a brief centrifugation at 8000 × g to pellet the beads, excess buffer was removed with a 27-gauge needle. The beads were washed with 100 volumes of the incubation buffer, excess liquid was removed, and the beads were resuspended in 20 μl of SDS-PAGE sample buffer. The samples were heated at 80 °C for 3 min and resolved by 180:1 acrylamide/bisacrylamide 14% SDS-PAGE. Protein bands were visualized by silver staining and analyzed by densitometry as described above. Chicken breast muscle expresses exclusively acidic TnT and mainly the α-isoform of Tm whereas chicken gastrocnemius muscle expresses basic TnT and α/β-Tm (25Ogut O. Granzier H. Jin J.-P. Am. J. Physiol. 1999; 276: C1162-1170Crossref PubMed Google Scholar). These muscles provide convenient sources for the purification of the protein isoforms. The TnT and Tm isoforms purified from these muscles are shown in Fig. 1. Chicken fast skeletal muscle TnI purified from the adult skeletal muscles was pooled together. The proteins are free of degradation but more importantly are free of other thin filament proteins as demonstrated by Western blotting using specific mAbs (Fig. 1). By densitometry, the ratio of α- to β-Tm was 9.71:1 for the α/α-Tm preparation and 1.15:1 for the α/β-Tm preparation. Indirect ELISA was done to determine the relative affinity of mAbs 3E4 and 6B8 toward the two classes of purified chicken fast skeletal muscle TnT. Both acidic and basic TnT were identified by 3E4 without significant differences in the titration curves (Fig.2 A). Because 3E4 detects TnT isoforms with equal relative affinity, it was suitable for comparing the interaction of acidic and basic TnT with TnI and Tm. In contrast, indirect ELISA titration showed that mAb 6B8 was highly selective for acidic TnT over basic TnT (Fig. 2 B). The two antibodies allow for either comparable (mAb 3E4) or selective (mAb 6B8) detection of chicken fast skeletal muscle TnT isoforms. Affinity chromatography was used to determine the relative binding affinity of acidic and basic TnT to Tm. As shown by the chromatographic profile (Fig. 3), both acidic and basic TnT bound specifically to the α/β-Tm affinity column with overlapping elution profiles. However, elution of basic TnT (peak at 312–325 mm NaCl) from the column slightly preceded acidic TnT (peak at 337–350 mm NaCl), suggesting that the relative binding affinity of acidic TnT for α/β-Tm was higher. Solid phase protein binding assay was also used to monitor the interactions between acidic or basic TnT isoforms with α/α- or α/β-Tm dimers. In this independent assay, the relative affinities of TnT isoforms for Tm isoforms, as defined by the TnT concentration required for 50% maximal binding, were not significantly different (Fig. 4; TableI). However, the saturation level of the binding curves between acidic TnT and Tm isoforms was significantly higher than for basic TnT (p < 0.05). The maximal binding of TnT to α/β-Tm was higher than binding to α/α-Tm. This occurred in the absence of statistically significant differences in the relative binding affinities as reflected by the TnT concentrations required for 50% maximal binding (Table I). With affinity chromatography, the initial TnT-Tm binding was saturated, and the affinity was compared through a salt gradient elution under non-equilibrium conditions. In contrast, the solid phase ELISA binding experiments measured the K d of the initial binding at equilibrium, which determined the shape of the titration curve and the concentration needed for 50% maximal binding. In addition, the absolute value of the binding curve was obtained after washing under non-equilibrium conditions, which appeared to be a more sensitive measurement of the binding affinity similar to that determined by the elution profile from an affinity column.Table IThe concentration of TnT required for 50% maximal binding to TnI or TmAcidic TnTBasic TnTμmα/α-Tm0.083 ± 0.0470.109 ± 0.014α/β-Tm0.128 ± 0.0210.076 ± 0.030TnI0.068 ± 0.0190.095 ± 0.009 Open table in a new tab Using the solid phase binding assay, the relative affinity of acidic or basic TnT for fast skeletal muscle TnI was determined. The titration curves for TnI binding (Fig. 5) demonstrate that the maximal binding of basic TnT to TnI was significantly higher than the binding of acidic TnT to TnI (p < 0.05). Similar to the Tnt-Tm interactions (Fig.4), no statistical difference in the relative affinities of the TnT isoforms for TnI was detected by the concentrations of TnT required to achieve 50% maximal binding (Table I). Troponin T isoform competition assays were performed to further characterize the different binding strengths of TnT isoforms to Tm. Instead of a simple competition between the two TnT isoforms, the results showed cooperativity between TnT isoforms for Tm binding. The initial increase of the concentration of basic TnT did not produce a competitive effect on the binding of acidic TnT. In contrast, between 25.0 and 75.0 nm total TnT in the assay (with molar ratios of basic/acidic TnT between 0 and 2), the binding of acidic TnT to α/α− and α/β-Tm was significantly increased (Fig.6). These results indicate that the binding of basic TnT to Tm promoted the binding of acidic TnT to Tm by a cooperative effect in the absence of F-actin. As expected, the binding of acidic TnT to Tm rapidly decreased at higher molar ratios of basic to acidic TnT, reflecting effective competition by the excess basic TnT. In similar competition experiments analyzing the binding of TnT isoforms to immobilized TnI, no such cooperative effect was observed. Increasing concentrations of basic TnT effectively competed with acidic TnT for TnI binding (Fig. 7). The competition experiments were done with two concentrations of TnI during coating (1 and 5 μg/ml) to ensure that the coating density of TnI was not responsible for the absence of cooperativity. Basic TnT competed more effectively with acidic TnT for TnI binding when 1 μg/ml TnI was used during coating versus 5 μg/ml. Unlike the cooperativity seen between TnT isoforms for Tm binding (Fig. 6), the interaction of TnT isoforms with TnI was strictly competitive. The cooperativity between TnT isoforms for Tm binding was independently shown by co-immunoprecipitation experiments. Using a constant concentration of acidic chicken fast skeletal muscle TnT (0.1 μm), Fig.8 A shows that increasing concentrations of up to 0.025 μm bovine cardiac TnT promoted cooperative binding of the fast skeletal muscle TnT to Tm. This resulted in an increase in the apparent affinity of fast skeletal muscle TnT for Tm. As the concentration of bovine cardiac TnT was further increased (0.025–0.1 μm), the fast skeletal muscle TnT was effectively competed from binding Tm. The cooperative Tm binding between cardiac and fast skeletal muscle TnT was also seen in the solid phase protein binding experiment (Fig. 8 B), similar to the cooperativity observed between the acidic and basic fast skeletal muscle TnT (Fig. 6). The relative binding of skeletal and cardiac TnT at equal concentrations (0.1 μm) showed that the affinity of cardiac TnT for Tm was higher (Fig. 8 A), consistent with a more effective cooperative effect of cardiac TnT than that of basic fast TnT on the binding of acidic fast TnT. Densitometry analyses showed that under the co-immunoprecipitation conditions, binding stoichiometry between TnT and Tm dimers was less than 1, indicating that the TnT-Tm interaction remained within physiological levels. To further dissect the basis for the cooperative binding between TnT and Tm, we repeated the solid phase competition experiments using TnT fragments. The NH2-terminal N165 fragment was unable to promote acidic TnT binding to Tm (Fig.9), suggesting that the central Tm binding site and the hypervariable NH2 terminus of TnT do not directly promote the cooperative interaction of TnT with Tm. However, the chymotryptic TnT fragment from the well conserved COOH-terminal region of TnT (T2 fragment) resulted in increased binding of acidic TnT to Tm. Therefore, the COOH-terminal Tm binding site of TnT alone may be the primary determinant to initiate in the cooperative binding of TnT to Tm. Isoform and sequence diversity of the NH2-terminal extension of TnT indicate that this fragment is a non-essential element. A genetically expressed TnT with an NH2-terminal truncation has been shown to conserve the core functions of full-length TnT (34Pan B.S. Gordon A.M. Potter J.D. J. Biol. Chem. 1991; 266: 12432-12438Abstract Full Text PDF PubMed Google Scholar). Therefore, the primary role of TnT in striated muscle thin filaments as an intermediary to tether TnI and TnC to Tm can be accomplished in the absence of the NH2terminus of TnT. Consistent with these observations, the highly conserved COOH-terminal region of TnT is responsible for TnI and TnC binding and has the higher affinity Tm binding site. The second Tm binding site resides in the central region of TnT (5Pearlstone J.R. Smillie L.B. Can. J. Biochem. Cell Biol. 1985; 63: 212-218Crossref PubMed Scopus (61) Google Scholar, 35Mak A.S. Smillie L.B. J. Mol. Biol. 1981; 149: 541-550Crossref PubMed Scopus (119) Google Scholar). However, an increasing amount of data demonstrates the functional importance of the variable NH2-terminal region of TnT (36Potter J.D. Sheng Z. Pan B.S. Zhao J. J. Biol. Chem. 1995; 270: 2557-2562Abstract Full Text Full Text PDF PubMed Scopus (141) Google Scholar, 37Malnic B. Farah C.S. Reinach F.C. J. Biol. Chem. 1998; 273: 10594-10601Abstract Full Text Full Text PDF PubMed Scopus (94) Google Scholar, 38Hinkle A. Goranson A. Butters C.A. Tobacman L.S. J. Biol. Chem. 1999; 274: 7157-7164Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar). This domain overlaps the head-to-tail junction of adjacent Tm dimers (39Brisson J.R. Golosinska K. Smillie L.B. Sykes B.D. Biochemistry. 1986; 25: 4548-4555Crossref PubMed Scopus (39) Google Scholar). Variations in the NH2-terminal primary structure are able to modify the overall conformation of TnT (27Wang J. Jin J.-P. Biochemistry. 1998; 37: 14519-14528Crossref PubMed Scopus (75) Google Scholar, 28Ogut O. Jin J.-P. Biochemistry. 1996; 35: 16581-16590Crossref PubMed Scopus (38) Google Scholar), affecting its interaction with Tm (29Ogut O. Jin J.-P. J. Biol. Chem. 1998; 273: 27858-27866Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar) and TnI (27Wang J. Jin J.-P. Biochemistry. 1998; 37: 14519-14528Crossref PubMed Scopus (75) Google Scholar) while changing the Ca2+ sensitivity of force and stiffness in skinned skeletal muscle fibers (25Ogut O. Granzier H. Jin J.-P. Am. J. Physiol. 1999; 276: C1162-1170Crossref PubMed Google Scholar). The effects of TnT isoforms on muscle contractility are likely to be mediated by changes in TnT interaction with other thin filament constituents. In this respect, the solid phase binding assay in Fig. 4 revealed a significant difference in the binding of acidicversus basic TnT to Tm. These variations in acidic and basic TnT binding to Tm must be attributed to the variable NH2-terminal region. This conclusion is supported by the observation that both the central and COOH-terminal regions of TnT are well conserved among species and identical in the chicken TnT compared in this study (29Ogut O. Jin J.-P. J. Biol. Chem. 1998; 273: 27858-27866Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). Therefore, the variable NH2 terminus of TnT must affect the tertiary structure of the central and/or COOH-terminal Tm binding sites. The role of TnT isoforms in contractility may also be defined by the differential binding of TnT isoforms to TnI (Fig. 5), an effect mediated through a single binding site near the COOH terminus of TnT (40Pearlstone J.R. Smillie L.B. J. Biol. Chem. 1983; 258: 2534-2542Abstract Full Text PDF PubMed Google Scholar). The differences in TnI binding between the acidic and basic TnT isoforms supports the conclusion that changes in the primary structure of the NH2-terminal variable region dictate changes in the overall tertiary structure of the TnT, including the functionally important COOH-terminal domain. Although the variable NH2-terminal region of TnT does not directly interact with TnI or Tm, its effects on the binding of intact TnT to these thin filament proteins is evident. In the solid phase competition assays (Fig. 6), basic TnT required a molar excess to effectively compete with acidic TnT most likely because of its weaker binding to Tm (Fig. 4). However, with an optimal range of concentration of total TnT in the reaction, the addition of basic TnT was able to increase the binding of acidic TnT to Tm by a cooperative effect. This was also shown in the co-immunoprecipitation experiments (Fig. 8) wherein bovine cardiac TnT increased the apparent affinity of acidic TnT for Tm. The cooperativity between TnT molecules for Tm binding appears to be a general feature because chicken basic fast TnT, bovine cardiac TnT, and the conserved COOH-terminal T2 fragment all induced cooperative binding of the acidic fast TnT to Tm. However, we have not excluded the possibility that the presence of two or more TnT isoforms (e.g. in most vertebrate fast skeletal muscles) may enhance the cooperative interaction with Tm. Although it has been previously shown that the troponin interaction with Tm-F-actin filaments is cooperative (38Hinkle A. Goranson A. Butters C.A. Tobacman L.S. J. Biol. Chem. 1999; 274: 7157-7164Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar), our experiments demonstrate that TnT-Tm cooperativity also exists in the absence of F-actin. Increased acidic TnT binding to Tm is well explained by cooperative recruitment of TnT molecules to adjacent Tm dimers following the initial interaction between TnT and Tm molecules. In effect, the binding of TnT to a Tm dimer would promote a conformational change not only in the Tm dimer bound to TnT but also in the adjacent naked Tm dimer. This conformational change would “prime” the adjacent Tm dimer for interaction with TnT, explaining the cooperativity seen in Figs. 6 and 8. Further, co-immunoprecipitation experiments demonstrated that the cooperative TnT-Tm interaction remained stoichiometric, mimicking the physiological ratio of one TnT molecule per Tm dimer. We found that the COOH-terminal Tm binding site of TnT was the initiator of the cooperative TnT-Tm interactions, as the COOH-terminal T2 fragment, not the NH2-terminal N165 fragment, promoted cooperative binding (Fig. 9). Because TnT from different genes (cardiac versusfast skeletal muscle) and species (avian versus mammalian) promoted cooperative TnT binding to Tm, this conserved function of TnT is expected to be mediated primarily by a conserved domain of the molecule. The importance of the T2 region of TnT in this cooperativity is intuitive, because it is the site of interaction for TnI-TnC and would be the primary intermediary following activation when TnC binds Ca2+. Because our detection of the cooperative TnT-Tm interaction was through the binding of intact TnT in the assay systems, we cannot exclude that the central region Tm binding site and the NH2-terminal domain of TnT contributed to the responding effect. Note that cooperative TnT binding to Tm in the presence of the T2 fragment was not as effective as in the presence of intact TnT. This is predicted and in agreement with previous data that the NH2-terminal variable region of TnT increases but is not critical to the cooperative binding of troponin to Tm-F-actin filaments (38Hinkle A. Goranson A. Butters C.A. Tobacman L.S. J. Biol. Chem. 1999; 274: 7157-7164Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar). However, our results suggest that association with F-actin is not absolutely required for the cooperativity and/or cross-talk between adjacent Tm dimers. The results suggest that there is a determinant of cooperativity in muscle activation/relaxation intrinsic to troponin-Tm interaction. Our experiments have demonstrated that acidic and basic TnT isoforms show distinct interactions within the thin filament. These changes in TnT function are predicted to be because of NH2-terminal variation-originated changes in the tertiary structure of the COOH-terminal T2 region where both TnI and Tm show strong interactions. However, the mechanism governing how functional differences in TnT isoforms contribute to muscle contractility remains unclear. Previous studies have demonstrated that striated muscle fibers expressing more acidic TnT isoforms show increased sensitivity to Ca2+ for both force and stiffness development (25Ogut O. Granzier H. Jin J.-P. Am. J. Physiol. 1999; 276: C1162-1170Crossref PubMed Google Scholar, 41Solaro R.J. Lee J.A. Kentish J.C. Allen D.G. Circ. Res. 1988; 63: 779-787Crossref PubMed Scopus (157) Google Scholar, 42Reiser P.J. Greaser M.L. Moss R.L. J. Physiol. ( Lond. ). 1992; 449: 573-588Crossref PubMed Scopus (62) Google Scholar). In addition, transgenic expression of a relatively basic fast skeletal muscle TnT in mouse hearts increased the cooperativity of the force response to Ca2+ (26Huang Q.-Q. Brozovich F.V. Jin J.-P. J. Physiol. ( Lond. ). 1999; 520 (a): 231-242Crossref PubMed Scopus (40) Google Scholar). These examples provide two outcomes of TnT isoform expression. At this point, we can only hypothesize the mechanism of contribution of TnT isoforms to these observations. It is well known that the most energetically favorable interaction in the myofilament assembly is the actin-myosin interaction. This is supported by the strong interaction of myosin heads with actin active sites during rigor in the absence of Ca2+ (for review, see Ref.43Cooke R. Physiol. Rev. 1997; 77: 671-697Crossref PubMed Scopus (247) Google Scholar). The troponin-Tm assembly serves to regulate this favored interaction through the cooperative release and/or return of the inhibition of cycling actomyosin cross-bridges. Therefore, a change in the cooperative interaction between TnT isoforms and Tm provides an effective point to regulate muscle contraction. We thank Dr. L. B. Smillie for the rabbit fast TnT-T2 fragment and helpful discussions and Dr. J. J.-C. Lin for the anti-Tm mAb CH1." @default.
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• W1964211232 title "Cooperative Interaction between Developmentally Regulated Troponin T and Tropomyosin Isoforms in the Absence of F-actin" @default.
• W1964211232 cites W1416357203 @default.
• W1964211232 cites W1492798688 @default.
• W1964211232 cites W1515802281 @default.
• W1964211232 cites W1536511686 @default.
• W1964211232 cites W1538939790 @default.
• W1964211232 cites W1540963282 @default.
• W1964211232 cites W1543087934 @default.
• W1964211232 cites W1554404149 @default.
• W1964211232 cites W1571231559 @default.
• W1964211232 cites W1597808722 @default.
• W1964211232 cites W1603479071 @default.
• W1964211232 cites W1620274191 @default.
• W1964211232 cites W1949811505 @default.
• W1964211232 cites W1969563350 @default.
• W1964211232 cites W1972994243 @default.
• W1964211232 cites W1973734209 @default.
• W1964211232 cites W1978484983 @default.
• W1964211232 cites W1983118029 @default.
• W1964211232 cites W1988986373 @default.
• W1964211232 cites W1995365778 @default.
• W1964211232 cites W1996858222 @default.
• W1964211232 cites W2007803373 @default.
• W1964211232 cites W2010536798 @default.
• W1964211232 cites W2021186617 @default.
• W1964211232 cites W2046296155 @default.
• W1964211232 cites W2054774765 @default.
• W1964211232 cites W2058552188 @default.
• W1964211232 cites W2061365077 @default.
• W1964211232 cites W2061820742 @default.
• W1964211232 cites W2065152211 @default.
• W1964211232 cites W2075568714 @default.
• W1964211232 cites W2083830165 @default.
• W1964211232 cites W2088958554 @default.
• W1964211232 cites W2095118899 @default.
• W1964211232 cites W2111732786 @default.
• W1964211232 cites W2121406342 @default.
• W1964211232 cites W2142232452 @default.
• W1964211232 cites W2147093254 @default.
• W1964211232 cites W2169410966 @default.
• W1964211232 cites W2175328062 @default.
• W1964211232 cites W2183214884 @default.
• W1964211232 cites W2210986612 @default.
• W1964211232 cites W80054755 @default.
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