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- W1964295364 abstract "Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histone proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in polyacrylamide gel in sodium dodecyl sulfate. Most chromatin non-histone proteins and nuclear phosphoproteins of pig lymph nodes have a molecular weight above 40 000 daltons and show a significant degree of homology of molecular range from 40 000 to 90 000 daltons. The phosphoproteins have been also analyzed by isoelectric focusing in polyacrylamide gel over the pH range of 3–10. They are distributed throughout the pI range of 5.8–9.0. The chromatin non-histone proteins and phosphoproteins are of acidic nature and contain 0,97 per cent tryptophan. A partir de la chromatine purifiée de nodules lymphatiques de porc, on a isolé les protéines chromosomales totales et la fraction contenant les protéines non-histones. Les phospho-protéines nucléaires sont extraites des noyaux isolés. Toutes les fractions protéiques sont comparées au moyen de l'électrophorèse analytique en gel de polyacrylamide en présence de SDS. Un grand nombre de protéines non-histones totales et de phospho-protéines nucléaires ont un poids moléculaire supérieur à 40 000 daltons et montrent un haut degré d'homologie pour les composants de poids moléculaire entre 40 000 et 90 000 daltons. Les phospho-protéines analysées par électrofocalisation montrent une distribution dans un intervalle de pH compris entre 5,8 et 9,0. Ces composants et les protéines non-histones totales se distinguent par leur caractère acide et leur teneur en tryptophane égale à 0,97 pour cent." @default.
- W1964295364 created "2016-06-24" @default.
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- W1964295364 date "1976-07-01" @default.
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- W1964295364 title "Electrophoretic studies of non-histone proteins from pig lymph nodes" @default.
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- W1964295364 doi "https://doi.org/10.1016/s0300-9084(76)80391-4" @default.
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