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- W1964315815 abstract "To elucidate the mechanism of the Ca(2+)-sensitizing action of pimobendan, cardiac thin filaments were reconstituted from actin and tropomyosin-troponin complex and made to slide on a myosin layer. Although filaments showed Brownian movement with a low Ca2+ concentration, they slid at a constant velocity above a certain level of Ca2+ concentration, showing that the sliding was regulated by Ca2+ within a narrow pCa range. Acidosis, addition of inorganic phosphate, and phosphorylation of troponin I increased the threshold Ca2+ concentration. Addition of pimobendan reversed these desensitization effects. These results clearly demonstrated that pimobendan directly increases the Ca2+ sensitivity of thin filament." @default.
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- W1964315815 date "1995-06-01" @default.
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- W1964315815 title "Pimobendan directly sensitizes reconstituted thin filament to slide on cardiac myosin" @default.
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- W1964315815 doi "https://doi.org/10.1016/0922-4106(95)90016-0" @default.
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