FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964346070> ?p ?o ?g. }

• W1964346070 endingPage "3165" @default.
• W1964346070 startingPage "3157" @default.
• W1964346070 abstract "The genomic RNA of potyvirases has a characteristic 5′ non-translated region (5′NTR) to which a viral protein, VPg, is covalently attached. This suggests that the viral RNA lacks a conventional cap structure and thus its translation may not proceed in the same way as most cellular mRNAs. To investigate the role of the 5′NTR during translation, various derivatives of the turnip mosaic potyvirus (TuMV) leader were fused to the reporter gene β-glucuronidase (GUS). These constructs were used to monitor the efficiency of translation in vitro in a rabbit reticulocyte lysate and in planta following microprojectile DNA delivery into tobacco cell suspensions. GUS transcripts fused with the TuMV 5′NTR, whether they were capped or not, were efficiently translated, whereas GUS transcripts without the viral leader needed to be capped for expression. When transcripts of the viral leader were supplied in excess over functional transcripts, translation was inhibited in a dose-dependent manner. Similarly, transcripts synthesized from the reverse complement of the 5′NTR inhibited translation to the same extent as the wild-type sequence, indicating that cap independence was not conferred by a specific sequence within the viral leader. A stable hairpin loop was placed in front or after the viral sequence. This hairpin loop normally prevented translation of control GUS transcripts but when the viral leader was positioned after it a significant level of GUS activity was measured, whether the transcripts were capped or not. On the other hand, when the hairpin loop was positioned after the viral leader, no GUS activity was measured. These results suggested that ribosomes bound to an internal site within the TuMV 5′NTR and then presumably scanned the sequence for the initiator AUG." @default.
• W1964346070 created "2016-06-24" @default.
• W1964346070 creator A5031902245 @default.
• W1964346070 creator A5040304020 @default.
• W1964346070 creator A5064761099 @default.
• W1964346070 creator A5074664109 @default.
• W1964346070 creator A5083387250 @default.
• W1964346070 date "1994-11-01" @default.
• W1964346070 modified "2023-10-16" @default.
• W1964346070 title "Evidence for an Internal Ribosome Entry Site Within the 5' Non-translated Region of Turnip Mosaic Potyvirus RNA" @default.
• W1964346070 cites W1539503448 @default.
• W1964346070 cites W1836032532 @default.
• W1964346070 cites W1840849116 @default.
• W1964346070 cites W1944327053 @default.
• W1964346070 cites W1975943688 @default.
• W1964346070 cites W1986408701 @default.
• W1964346070 cites W2005679114 @default.
• W1964346070 cites W2013923998 @default.
• W1964346070 cites W2014457420 @default.
• W1964346070 cites W2019455820 @default.
• W1964346070 cites W2021978074 @default.
• W1964346070 cites W2025763720 @default.
• W1964346070 cites W2026168006 @default.
• W1964346070 cites W2026218192 @default.
• W1964346070 cites W2027435532 @default.
• W1964346070 cites W2027817431 @default.
• W1964346070 cites W2030054987 @default.
• W1964346070 cites W2039335276 @default.
• W1964346070 cites W2039956664 @default.
• W1964346070 cites W2048731469 @default.
• W1964346070 cites W2062593578 @default.
• W1964346070 cites W2064034185 @default.
• W1964346070 cites W2065104935 @default.
• W1964346070 cites W2072962648 @default.
• W1964346070 cites W2078918891 @default.
• W1964346070 cites W2081969490 @default.
• W1964346070 cites W2098477883 @default.
• W1964346070 cites W2139568440 @default.
• W1964346070 cites W2144144425 @default.
• W1964346070 cites W2144634347 @default.
• W1964346070 cites W2159498974 @default.
• W1964346070 cites W2160980854 @default.
• W1964346070 cites W2165906919 @default.
• W1964346070 cites W2166661434 @default.
• W1964346070 cites W2168847939 @default.
• W1964346070 cites W2179196912 @default.
• W1964346070 cites W569951484 @default.
• W1964346070 cites W67546116 @default.
• W1964346070 doi "https://doi.org/10.1099/0022-1317-75-11-3157" @default.
• W1964346070 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7964625" @default.
• W1964346070 hasPublicationYear "1994" @default.
• W1964346070 type Work @default.
• W1964346070 sameAs 1964346070 @default.
• W1964346070 citedByCount "81" @default.
• W1964346070 countsByYear W19643460702012 @default.
• W1964346070 countsByYear W19643460702013 @default.
• W1964346070 countsByYear W19643460702014 @default.
• W1964346070 countsByYear W19643460702015 @default.
• W1964346070 countsByYear W19643460702016 @default.
• W1964346070 countsByYear W19643460702017 @default.
• W1964346070 countsByYear W19643460702018 @default.
• W1964346070 countsByYear W19643460702019 @default.
• W1964346070 countsByYear W19643460702020 @default.
• W1964346070 countsByYear W19643460702021 @default.
• W1964346070 countsByYear W19643460702022 @default.
• W1964346070 countsByYear W19643460702023 @default.
• W1964346070 crossrefType "journal-article" @default.
• W1964346070 hasAuthorship W1964346070A5031902245 @default.
• W1964346070 hasAuthorship W1964346070A5040304020 @default.
• W1964346070 hasAuthorship W1964346070A5064761099 @default.
• W1964346070 hasAuthorship W1964346070A5074664109 @default.
• W1964346070 hasAuthorship W1964346070A5083387250 @default.
• W1964346070 hasBestOaLocation W19643460701 @default.
• W1964346070 hasConcept C104317684 @default.
• W1964346070 hasConcept C105580179 @default.
• W1964346070 hasConcept C109110057 @default.
• W1964346070 hasConcept C149364088 @default.
• W1964346070 hasConcept C153911025 @default.
• W1964346070 hasConcept C159047783 @default.
• W1964346070 hasConcept C2522874641 @default.
• W1964346070 hasConcept C2776625791 @default.
• W1964346070 hasConcept C2777806494 @default.
• W1964346070 hasConcept C4718897 @default.
• W1964346070 hasConcept C54355233 @default.
• W1964346070 hasConcept C67705224 @default.
• W1964346070 hasConcept C77430603 @default.
• W1964346070 hasConcept C86803240 @default.
• W1964346070 hasConcept C88478588 @default.
• W1964346070 hasConceptScore W1964346070C104317684 @default.
• W1964346070 hasConceptScore W1964346070C105580179 @default.
• W1964346070 hasConceptScore W1964346070C109110057 @default.
• W1964346070 hasConceptScore W1964346070C149364088 @default.
• W1964346070 hasConceptScore W1964346070C153911025 @default.
• W1964346070 hasConceptScore W1964346070C159047783 @default.
• W1964346070 hasConceptScore W1964346070C2522874641 @default.
• W1964346070 hasConceptScore W1964346070C2776625791 @default.
• W1964346070 hasConceptScore W1964346070C2777806494 @default.
• W1964346070 hasConceptScore W1964346070C4718897 @default.

• next