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- W1964360147 abstract "Marrow from five donors was aspirated into a small volume of heparinized culture media, filtered, and stored in 10-ml nominal plastic bags at 4, 22, and 37°C, respectively, for 7 days with and without agitation. Samples were taken at 0, 1, 2, 3, and 7 days, respectively, for determination of cell numbers, trypan blue viability, and committed colony-forming unit-granulocyte-monocyte (CFU-GM) and erythroid burst-forming unit (BFU-E) and colony-forming unit (CFU-E), as well as multipotent (CFU-MIX), hematopoietic progenitors. Glucose and pH decreased with storage, most markedly at higher storage temperatures. Recovery of recovered cells exceeded 90 percent under all conditions at 7 days. Viability of committed and multipotent progenitors was maintained fairly well for 3 days at 4 and 22°C, but fell off rapidly after 1 day at 37°C. Recovery of progenitor cells stored 7 days was relatively poor. Best maintenance was achieved for 3 days using 4°C storage without agitation." @default.
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- W1964360147 date "1986-07-01" @default.
- W1964360147 modified "2023-10-12" @default.
- W1964360147 title "Liquid storage of unseparated human bone marrow. Evaluation of hematopoietic progenitors by clonal assay" @default.
- W1964360147 doi "https://doi.org/10.1046/j.1537-2995.1986.26486262739.x" @default.
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