Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964433194> ?p ?o ?g. }
- W1964433194 endingPage "18624" @default.
- W1964433194 startingPage "18614" @default.
- W1964433194 abstract "P13 is one of the major outer membrane proteins of Borrelia burgdorferi. Previous studies described P13 as a porin. In the present study some structure and function aspects of P13 were studied. P13 showed according to lipid bilayer studies a channel-forming activity of 0.6 nanosiemens in 1 m KCl. Single channel and selectivity measurements demonstrated that P13 had no preference for either cations or anions and showed no voltage-gating up to ±100 mV. Blue native polyacrylamide gel electrophoresis was used to isolate and characterize the P13 protein complex in its native state. The complex had a high molecular mass of about 300 kDa and was only composed of P13 monomers. The channel size was investigated using non-electrolytes revealing an apparent diameter of about 1.4 nm with a 400-Da molecular mass cut-off. Multichannel titrations with different substrates reinforced the idea that P13 forms a general diffusion channel. The identity of P13 within the complex was confirmed by second dimension SDS-PAGE, Western blotting, mass spectrometry, and the use of a p13 deletion mutant strain. The results suggested that P13 is the protein responsible for the 0.6-nanosiemens pore-forming activity in the outer membrane of B. burgdorferi." @default.
- W1964433194 created "2016-06-24" @default.
- W1964433194 creator A5003781018 @default.
- W1964433194 creator A5017531068 @default.
- W1964433194 creator A5025063833 @default.
- W1964433194 creator A5041718533 @default.
- W1964433194 creator A5042367226 @default.
- W1964433194 creator A5050815650 @default.
- W1964433194 creator A5061353923 @default.
- W1964433194 creator A5069862319 @default.
- W1964433194 creator A5070561732 @default.
- W1964433194 date "2014-07-01" @default.
- W1964433194 modified "2023-10-02" @default.
- W1964433194 title "Study of the Protein Complex, Pore Diameter, and Pore-forming Activity of the Borrelia burgdorferi P13 Porin" @default.
- W1964433194 cites W1582854101 @default.
- W1964433194 cites W1857832081 @default.
- W1964433194 cites W1880959843 @default.
- W1964433194 cites W1882462500 @default.
- W1964433194 cites W1926303070 @default.
- W1964433194 cites W1963530308 @default.
- W1964433194 cites W1965055845 @default.
- W1964433194 cites W1966103266 @default.
- W1964433194 cites W1970898100 @default.
- W1964433194 cites W1973369594 @default.
- W1964433194 cites W1981267913 @default.
- W1964433194 cites W1987148981 @default.
- W1964433194 cites W1988901585 @default.
- W1964433194 cites W1997698750 @default.
- W1964433194 cites W2008498696 @default.
- W1964433194 cites W2018046614 @default.
- W1964433194 cites W2019074098 @default.
- W1964433194 cites W2021793865 @default.
- W1964433194 cites W2025279524 @default.
- W1964433194 cites W2028409160 @default.
- W1964433194 cites W2031977066 @default.
- W1964433194 cites W2035003519 @default.
- W1964433194 cites W2044853699 @default.
- W1964433194 cites W2047635824 @default.
- W1964433194 cites W2055155126 @default.
- W1964433194 cites W2057572812 @default.
- W1964433194 cites W2062885416 @default.
- W1964433194 cites W2063367477 @default.
- W1964433194 cites W2066815397 @default.
- W1964433194 cites W2068140507 @default.
- W1964433194 cites W2068259161 @default.
- W1964433194 cites W2069449656 @default.
- W1964433194 cites W2072738851 @default.
- W1964433194 cites W2073445575 @default.
- W1964433194 cites W2076567649 @default.
- W1964433194 cites W2080147524 @default.
- W1964433194 cites W2084676771 @default.
- W1964433194 cites W2088668571 @default.
- W1964433194 cites W2109469926 @default.
- W1964433194 cites W2114967331 @default.
- W1964433194 cites W2120019901 @default.
- W1964433194 cites W2120061656 @default.
- W1964433194 cites W2120521437 @default.
- W1964433194 cites W2145082457 @default.
- W1964433194 cites W2146450962 @default.
- W1964433194 cites W2147136083 @default.
- W1964433194 cites W2147987529 @default.
- W1964433194 cites W2170455757 @default.
- W1964433194 cites W2324443543 @default.
- W1964433194 cites W267625338 @default.
- W1964433194 cites W4211022623 @default.
- W1964433194 cites W4239494812 @default.
- W1964433194 doi "https://doi.org/10.1074/jbc.m113.539528" @default.
- W1964433194 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4081907" @default.
- W1964433194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24825899" @default.
- W1964433194 hasPublicationYear "2014" @default.
- W1964433194 type Work @default.
- W1964433194 sameAs 1964433194 @default.
- W1964433194 citedByCount "14" @default.
- W1964433194 countsByYear W19644331942015 @default.
- W1964433194 countsByYear W19644331942016 @default.
- W1964433194 countsByYear W19644331942017 @default.
- W1964433194 countsByYear W19644331942018 @default.
- W1964433194 countsByYear W19644331942019 @default.
- W1964433194 countsByYear W19644331942022 @default.
- W1964433194 crossrefType "journal-article" @default.
- W1964433194 hasAuthorship W1964433194A5003781018 @default.
- W1964433194 hasAuthorship W1964433194A5017531068 @default.
- W1964433194 hasAuthorship W1964433194A5025063833 @default.
- W1964433194 hasAuthorship W1964433194A5041718533 @default.
- W1964433194 hasAuthorship W1964433194A5042367226 @default.
- W1964433194 hasAuthorship W1964433194A5050815650 @default.
- W1964433194 hasAuthorship W1964433194A5061353923 @default.
- W1964433194 hasAuthorship W1964433194A5069862319 @default.
- W1964433194 hasAuthorship W1964433194A5070561732 @default.
- W1964433194 hasBestOaLocation W19644331941 @default.
- W1964433194 hasConcept C101660505 @default.
- W1964433194 hasConcept C104317684 @default.
- W1964433194 hasConcept C116084860 @default.
- W1964433194 hasConcept C12554922 @default.
- W1964433194 hasConcept C144647389 @default.
- W1964433194 hasConcept C146587185 @default.
- W1964433194 hasConcept C159654299 @default.
- W1964433194 hasConcept C166940927 @default.