FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964433774> ?p ?o ?g. }

• W1964433774 endingPage "1119" @default.
• W1964433774 startingPage "1114" @default.
• W1964433774 abstract "돼지 정액의 세균오염은 정자활력 감소나 수태율 저하 등을 유발하는데, 특히 Stenotrophomonas maltophilia는 자연환경에 널리 존재하여 비위생적으로 채취된 정액에 많이 오염될 뿐 아니라 사람에게도 병원성을 일으킨다. 본 연구에서는 S. maltophilia의 검사 시간 단축과 정확도를 높이고자 PCR 기법을 개발하였으며, 돼지 원정액에서 이들 세균의 오염율을 조사하고 유효 항생제를 선발하고자 하였다. 돼지 원정액에서 분리 빈도가 높은 18 균종을 대상으로 PCR을 적용한 결과, S. maltophilia에서만 445 bp의 특이 유전자 증폭산물이 확인되었다. 또한 순수 배양된 S. maltophilia는 <TEX>$1.5{times}10^3$</TEX> CFU/ml까지, 원정액에 혼합된 S. maltophilia에 대해서는 <TEX>$1.5{times}10^4$</TEX> CFU/ml까지 검출이 가능하였다. 2009년에 116건과 2011년 324건 등 총 440건의 원정액 중 26건(5.9%)에서 S. maltophilia가 분리되었으며, 여름과 가을철에 오염율이 상대적으로 높게 나타났다. 균분리법과 PCR간의 일치율은 98.9%, kappa value는 0.906이었으며, PCR의 민감도와 특이도는 각각 100%, 98.7%로 나타났다. 분리균주의 항생제 감수성 시험 결과, enrofloxacin (100%)과 florfenicol (92.3%)에 높은 감수성을 보였으나 amoxicillin/clavulanic acid, apramycin, ceftiofur, penicillin, spectinomycin에는 내성율이 높게 나타났다. 결론적으로 개발된 PCR기법은 민감도, 특이도, 일치율이 높아 균분리법을 보조하고 신속 정확하게 정액 내 오염세균을 확인할 수 있어 효율적인 정액관리가 가능할 것으로 기대된다. Bacteria are frequently contaminated during the collection and processing procedures of boar semen. Of the contaminants, Stenotrophomonas (S.) maltophilia is a Gram-negative bacterium that is widely distributed in a variety of habitats. Although PCR assays have been developed for the detection of S. maltophilia, they cross-react with some species of Xanthomonas. In this study, we designed a primer set for the detection of S. maltophilia in order to target the chiA (GenBank accession no. NC_010943) gene. The specific PCR products were amplified from S. maltophilia only, not from other tested strains that are frequently found in semen. The detection limit of the PCR was <TEX>$1.5{times}10^3$</TEX> CFU/ml with pure-cultured S. maltophilia and <TEX>$1.5{times}10^4$</TEX> CFU/ml with S. maltophilia spiked in semen. Twenty-six (5.9%) S. maltophilia were isolated from 440 semen samples. The PCR results exhibited 98.9% agreement with a comparison of S. maltophilia isolation. Also, the sensitivity and specificity of the PCR were 100% and 98.7%, respectively. In the antimicrobial susceptibility test, S. maltophilia isolates were highly susceptible to enrofloxacin and florfenicol, while the majority of them were resistant to amoxicillin/clavulanic acid, apramycin, ceftiofur, penicillin, and spectinomycin. These results indicated that the PCR using the chiA gene was proven to be reliable and effective for the detection of S. maltophilia with high levels of sensitivity and specificity." @default.
• W1964433774 created "2016-06-24" @default.
• W1964433774 creator A5000632630 @default.
• W1964433774 creator A5023487389 @default.
• W1964433774 creator A5033393093 @default.
• W1964433774 creator A5048621587 @default.
• W1964433774 creator A5049596211 @default.
• W1964433774 creator A5053528285 @default.
• W1964433774 creator A5059285472 @default.
• W1964433774 creator A5063020261 @default.
• W1964433774 date "2012-08-30" @default.
• W1964433774 modified "2023-10-05" @default.
• W1964433774 title "Establishment of PCR Conditions for the Identification of Stenotrophomonas maltophilia Isolated from Boar Semen and Antimicrobial Susceptibility Patterns of the Isolates" @default.
• W1964433774 cites W1851388668 @default.
• W1964433774 cites W1978505691 @default.
• W1964433774 cites W2044048024 @default.
• W1964433774 cites W2045822401 @default.
• W1964433774 cites W2051602281 @default.
• W1964433774 cites W2054462603 @default.
• W1964433774 cites W2055258502 @default.
• W1964433774 cites W2059828302 @default.
• W1964433774 cites W2060674920 @default.
• W1964433774 cites W2066762293 @default.
• W1964433774 cites W2090581372 @default.
• W1964433774 cites W2091774677 @default.
• W1964433774 cites W2100285072 @default.
• W1964433774 cites W2136365752 @default.
• W1964433774 cites W2136502185 @default.
• W1964433774 cites W2136931384 @default.
• W1964433774 cites W2139279951 @default.
• W1964433774 cites W2145797363 @default.
• W1964433774 cites W2148539042 @default.
• W1964433774 cites W2165370876 @default.
• W1964433774 cites W2245375342 @default.
• W1964433774 cites W2247858607 @default.
• W1964433774 cites W269313351 @default.
• W1964433774 cites W9345016 @default.
• W1964433774 cites W3125513499 @default.
• W1964433774 doi "https://doi.org/10.5352/jls.2012.22.8.1114" @default.
• W1964433774 hasPublicationYear "2012" @default.
• W1964433774 type Work @default.
• W1964433774 sameAs 1964433774 @default.
• W1964433774 citedByCount "8" @default.
• W1964433774 countsByYear W19644337742012 @default.
• W1964433774 crossrefType "journal-article" @default.
• W1964433774 hasAuthorship W1964433774A5000632630 @default.
• W1964433774 hasAuthorship W1964433774A5023487389 @default.
• W1964433774 hasAuthorship W1964433774A5033393093 @default.
• W1964433774 hasAuthorship W1964433774A5048621587 @default.
• W1964433774 hasAuthorship W1964433774A5049596211 @default.
• W1964433774 hasAuthorship W1964433774A5053528285 @default.
• W1964433774 hasAuthorship W1964433774A5059285472 @default.
• W1964433774 hasAuthorship W1964433774A5063020261 @default.
• W1964433774 hasBestOaLocation W19644337741 @default.
• W1964433774 hasConcept C2776352461 @default.
• W1964433774 hasConcept C2777637488 @default.
• W1964433774 hasConcept C2780663544 @default.
• W1964433774 hasConcept C501593827 @default.
• W1964433774 hasConcept C523546767 @default.
• W1964433774 hasConcept C54355233 @default.
• W1964433774 hasConcept C86803240 @default.
• W1964433774 hasConcept C89423630 @default.
• W1964433774 hasConceptScore W1964433774C2776352461 @default.
• W1964433774 hasConceptScore W1964433774C2777637488 @default.
• W1964433774 hasConceptScore W1964433774C2780663544 @default.
• W1964433774 hasConceptScore W1964433774C501593827 @default.
• W1964433774 hasConceptScore W1964433774C523546767 @default.
• W1964433774 hasConceptScore W1964433774C54355233 @default.
• W1964433774 hasConceptScore W1964433774C86803240 @default.
• W1964433774 hasConceptScore W1964433774C89423630 @default.
• W1964433774 hasIssue "8" @default.
• W1964433774 hasLocation W19644337741 @default.
• W1964433774 hasOpenAccess W1964433774 @default.
• W1964433774 hasPrimaryLocation W19644337741 @default.
• W1964433774 hasRelatedWork W1493576436 @default.
• W1964433774 hasRelatedWork W1971881372 @default.
• W1964433774 hasRelatedWork W1974975095 @default.
• W1964433774 hasRelatedWork W1994979904 @default.
• W1964433774 hasRelatedWork W2010935573 @default.
• W1964433774 hasRelatedWork W2033662520 @default.
• W1964433774 hasRelatedWork W2091983121 @default.
• W1964433774 hasRelatedWork W2366318833 @default.
• W1964433774 hasRelatedWork W2610563426 @default.
• W1964433774 hasRelatedWork W4240874719 @default.
• W1964433774 hasVolume "22" @default.
• W1964433774 isParatext "false" @default.
• W1964433774 isRetracted "false" @default.
• W1964433774 magId "1964433774" @default.
• W1964433774 workType "article" @default.