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• W1964437900 endingPage "5207" @default.
• W1964437900 startingPage "5194" @default.
• W1964437900 abstract "HP0268 is a conserved, uncharacterized protein from Helicobacter pylori. Here, we determined the solution structure of HP0268 using three-dimensional nuclear magnetic resonance (NMR) spectroscopy, revealing that this protein is structurally most similar to a small MutS-related (SMR) domain that exhibits nicking endonuclease activity. We also demonstrated for the first time that HP0268 is a nicking endonuclease and a purine-specific ribonuclease through gel electrophoresis and fluorescence spectroscopy. The nuclease activities for DNA and RNA were maximally increased by Mn(2+) and Mg(2+) ions, respectively, and decreased by Cu(2+) ions. Using NMR chemical shift perturbations, the metal and nucleotide binding sites of HP0268 were determined to be spatially divided but close to each other. The lysine residues (Lys7, Lys11 and Lys43) are clustered and form the nucleotide binding site. Moreover, site-directed mutagenesis was used to define the catalytic active site of HP0268, revealing that this site contains two acidic residues, Asp50 and Glu54, in the metal binding site. The nucleotide binding and active sites are not conserved in the structural homologues of HP0268. This study will contribute to improving our understanding of the structure and functionality of a wide spectrum of nucleases." @default.
• W1964437900 created "2016-06-24" @default.
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• W1964437900 date "2015-04-27" @default.
• W1964437900 modified "2023-09-26" @default.
• W1964437900 title "Structure-based functional identification of Helicobacter pylori HP0268 as a nuclease with both DNA nicking and RNase activities" @default.
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• W1964437900 doi "https://doi.org/10.1093/nar/gkv348" @default.
• W1964437900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4446426" @default.
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• W1964437900 hasPublicationYear "2015" @default.
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