FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964442246> ?p ?o ?g. }

• W1964442246 endingPage "2205" @default.
• W1964442246 startingPage "2198" @default.
• W1964442246 abstract "Many mutant variants of the P22 Arc repressor are subject to intracellular proteolysis in Escherichia coli, which precludes their expression at levels sufficient for purification and subsequent biochemical characterization. Here we examine the effects of several different C-terminal extension sequences on the expression and activity of a set of Arc mutants. We show that two tail sequences, KNQHE (st5) and H6KNQHE (st11), increase the expression levels of most mutants from 10- to 20-fold and, in some cases, result in restoration of biological activity in the cell. A third tail sequence, HHHHHH (st6), was not as effective in increasing mutant expression levels. All three tail sequences are functionally and structurally silent, as judged by their lack of effects on the DNA binding activity and stability of otherwise wild-type Arc. The properties of the st11 tail sequence make it an efficient system for the expression and purification of mutant Arc proteins, both because mutant expression levels are increased and because the proteins can be rapidly purified using nickel-chelate affinity chromatography. Arc mutants containing the EA28, RL31, and SA32 mutations were purified in the st11 background. The thermodynamic stability of the EA28 mutant (ΔΔGu -0.4 kcal/mol) is reduced modestly compared to the st11 parent, whereas the RL31 mutant (ΔΔGu -3.0 kcal/mol) and SA32 mutant (ΔΔGu -3.3 kcal/mol) are substantially less stable." @default.
• W1964442246 created "2016-06-24" @default.
• W1964442246 creator A5011979076 @default.
• W1964442246 creator A5015001132 @default.
• W1964442246 creator A5048665391 @default.
• W1964442246 date "1993-12-01" @default.
• W1964442246 modified "2023-09-25" @default.
• W1964442246 title "P22 Arc repressor: Enhanced expression of unstable mutants by addition of polar C-terminal sequences" @default.
• W1964442246 cites W1485218690 @default.
• W1964442246 cites W1519258564 @default.
• W1964442246 cites W1820160793 @default.
• W1964442246 cites W1975085160 @default.
• W1964442246 cites W1985842673 @default.
• W1964442246 cites W2000610610 @default.
• W1964442246 cites W2012672413 @default.
• W1964442246 cites W2018289835 @default.
• W1964442246 cites W2021385148 @default.
• W1964442246 cites W2023577097 @default.
• W1964442246 cites W2036384511 @default.
• W1964442246 cites W2043370748 @default.
• W1964442246 cites W2049378642 @default.
• W1964442246 cites W2066963442 @default.
• W1964442246 cites W2070543174 @default.
• W1964442246 cites W2091647812 @default.
• W1964442246 cites W2092095217 @default.
• W1964442246 cites W2094692963 @default.
• W1964442246 cites W2100413315 @default.
• W1964442246 cites W2100544480 @default.
• W1964442246 cites W2100837269 @default.
• W1964442246 cites W2101117567 @default.
• W1964442246 cites W2158649428 @default.
• W1964442246 cites W2167223768 @default.
• W1964442246 doi "https://doi.org/10.1002/pro.5560021219" @default.
• W1964442246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2142328" @default.
• W1964442246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8298465" @default.
• W1964442246 hasPublicationYear "1993" @default.
• W1964442246 type Work @default.
• W1964442246 sameAs 1964442246 @default.
• W1964442246 citedByCount "62" @default.
• W1964442246 countsByYear W19644422462012 @default.
• W1964442246 countsByYear W19644422462013 @default.
• W1964442246 countsByYear W19644422462016 @default.
• W1964442246 countsByYear W19644422462018 @default.
• W1964442246 countsByYear W19644422462021 @default.
• W1964442246 crossrefType "journal-article" @default.
• W1964442246 hasAuthorship W1964442246A5011979076 @default.
• W1964442246 hasAuthorship W1964442246A5015001132 @default.
• W1964442246 hasAuthorship W1964442246A5048665391 @default.
• W1964442246 hasBestOaLocation W19644422461 @default.
• W1964442246 hasConcept C104317684 @default.
• W1964442246 hasConcept C143065580 @default.
• W1964442246 hasConcept C150194340 @default.
• W1964442246 hasConcept C153911025 @default.
• W1964442246 hasConcept C158448853 @default.
• W1964442246 hasConcept C181199279 @default.
• W1964442246 hasConcept C2524010 @default.
• W1964442246 hasConcept C2779926675 @default.
• W1964442246 hasConcept C2781307694 @default.
• W1964442246 hasConcept C33923547 @default.
• W1964442246 hasConcept C501734568 @default.
• W1964442246 hasConcept C55493867 @default.
• W1964442246 hasConcept C83415579 @default.
• W1964442246 hasConcept C86803240 @default.
• W1964442246 hasConceptScore W1964442246C104317684 @default.
• W1964442246 hasConceptScore W1964442246C143065580 @default.
• W1964442246 hasConceptScore W1964442246C150194340 @default.
• W1964442246 hasConceptScore W1964442246C153911025 @default.
• W1964442246 hasConceptScore W1964442246C158448853 @default.
• W1964442246 hasConceptScore W1964442246C181199279 @default.
• W1964442246 hasConceptScore W1964442246C2524010 @default.
• W1964442246 hasConceptScore W1964442246C2779926675 @default.
• W1964442246 hasConceptScore W1964442246C2781307694 @default.
• W1964442246 hasConceptScore W1964442246C33923547 @default.
• W1964442246 hasConceptScore W1964442246C501734568 @default.
• W1964442246 hasConceptScore W1964442246C55493867 @default.
• W1964442246 hasConceptScore W1964442246C83415579 @default.
• W1964442246 hasConceptScore W1964442246C86803240 @default.
• W1964442246 hasIssue "12" @default.
• W1964442246 hasLocation W19644422461 @default.
• W1964442246 hasLocation W19644422462 @default.
• W1964442246 hasLocation W19644422463 @default.
• W1964442246 hasLocation W19644422464 @default.
• W1964442246 hasOpenAccess W1964442246 @default.
• W1964442246 hasPrimaryLocation W19644422461 @default.
• W1964442246 hasRelatedWork W1897859244 @default.
• W1964442246 hasRelatedWork W1968150876 @default.
• W1964442246 hasRelatedWork W1969822806 @default.
• W1964442246 hasRelatedWork W2024850895 @default.
• W1964442246 hasRelatedWork W2147466517 @default.
• W1964442246 hasRelatedWork W2380801951 @default.
• W1964442246 hasRelatedWork W2410546851 @default.
• W1964442246 hasRelatedWork W2414940753 @default.
• W1964442246 hasRelatedWork W249304337 @default.
• W1964442246 hasRelatedWork W3089217778 @default.
• W1964442246 hasVolume "2" @default.
• W1964442246 isParatext "false" @default.
• W1964442246 isRetracted "false" @default.
• W1964442246 magId "1964442246" @default.

• next