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• W1964478378 abstract "Abstract As a basis for embryo cryostorage research, information on relative toxicities of cryoprotectants is of key importance. Both morulae and tail-bud-stage embryos of red drum (Sciaenops ocellatus) were exposed to cryoprotectant solutions for 20 min at room temperature. Artificial seawater (ASW) at 30–32‰ was used as a medium. Embryos were exposed to solutions in a one-step addition. Toxicity was evaluated in terms of percentage hatch and 40-h survival. Maximum concentrations tolerated by morulae without significant reduction in hatch and survival were 1 M glycerol, 2 M dimethyl sulfoxide (DMSO), 2 M methanol, 0.5 M sucrose, and 95‰ ASW. Ethylene glycol was toxic to morulae even at 0.25 M. Tail-bud embryos were more resistant, with maximum innocuous concentrations being 1 M glycerol, 2 M DMSO, 2 M ethylene glycol, 2 M methanol, 0.5 M sucrose, and 95‰ ASW. Serial addition and dilution of 95‰ ASW offered no advantage over a direct plunge and rinse. Maximum harmless concentrations may not afford adequate cryoprotection. Reduced hatch and survival may be a result of osmotic shock or ionic imbalance rather than a direct consequence of toxicity. Preliminary cryopreservation experiments did not achieve survival of embryos cooled to subzero temperatures." @default.
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• W1964478378 date "1988-07-01" @default.
• W1964478378 modified "2023-10-18" @default.
• W1964478378 title "Toxicity of the Cryoprotectants Glycerol, Dimethyl Sulfoxide, Ethylene Glycol, Methanol, Sucrose, and Sea Salt Solutions to the Embryos of Red Drum" @default.
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• W1964478378 doi "https://doi.org/10.1577/1548-8640(1988)050<0148:totcgd>2.3.co;2" @default.
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