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- W1964552017 abstract "MicroRNAs (miRNAs) are excised from hairpin structures within primary miRNAs (pri-miRNAs). Most animal pri-miRNAs are processed by two cleavages, the first at a loop-distal site ∼11 nucleotides (nt) from the end of the hairpin and the second ∼22 nt beyond the first [1Lee Y. Jeon K. Lee J.T. Kim S. Kim V.N. MicroRNA maturation: Stepwise processing and subcellular localization.EMBO J. 2002; 21: 4663-4670Crossref PubMed Scopus (1703) Google Scholar, 2Lee R.C. Feinbaum R.L. Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.Cell. 1993; 75: 843-854Abstract Full Text PDF PubMed Scopus (9648) Google Scholar, 3Han J. Lee Y. Yeom K.H. Nam J.W. Heo I. Rhee J.K. Sohn S.Y. Cho Y. Zhang B.T. Kim V.N. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex.Cell. 2006; 125: 887-901Abstract Full Text Full Text PDF PubMed Scopus (1173) Google Scholar]. To identify RNA structural determinants of miRNA processing in plants, we analyzed the functional consequences of changing the secondary structure of the lower (loop-distal), middle (miRNA:miRNA∗), and upper (loop-proximal) stems of the hairpin in two different pri-miRNAs. Closing bulges immediately below the loop-distal cleavage sites increased the accumulation of accurately cleaved precursor miRNAs but decreased the abundance of the mature miRNAs. A pri-miRNA variant with an unpaired lower stem was not processed, and variants with a perfectly paired middle or upper stem were processed normally. Bioinformatic analysis of pri-miRNA structures, together with physical mapping of initial cleavage sites and in vitro processing of pri-miRNA, reveals that the first, loop-distal cleavage is often at a distance of ∼15 nt from an unpaired region. Hence, a common determinant of the rate and location of the initial pri-miRNA cleavage is an imperfectly base-paired duplex of ∼15 nt between the miRNA:miRNA∗ duplex and either a less structured region of the lower stem or its end." @default.
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- W1964552017 date "2010-01-01" @default.
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- W1964552017 title "RNA Secondary Structural Determinants of miRNA Precursor Processing in Arabidopsis" @default.
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- W1964552017 doi "https://doi.org/10.1016/j.cub.2009.10.076" @default.
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