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- W1964557478 abstract "The effect of different activation treatments on the activity of β-glucosidase and trehalase duringDictyostelium discoideum spore germination was examined. Spores were activated by the following methods: autoactivation, heat shock at 45°C for 30 minutes, 20% dimethyl sulfoxide (DMSO) for 30 minutes, and 8m urea for 30 min. After subjecting SG1 spores to the above treatments, the activities of β-glucosidase and trehalase were monitored. These enzymes normally increase in activity during the end of the germination process when spores are activated with heat treatment. Both enzymes exhibited temporal and quantitative shifts in activity which were dependent on the methods used for spore activation. The temporal and quantitative changes in trehalase activity were more pronounced than the changes in β-glucosidase activity. The specific activity of endogenous trehalase changed from a maximum of 4.5 in heat-activated spores to a minimum of 0.75 in autoactivated spores, with the increase in specific activity shifted from late swelling to early swelling. DMSO- and urea-induced activation resulted in the accumulation of trehalase during the middle of spore swelling with final endogenous activity values between 2 and 3. On the other hand, β-glucosidase showed only a slight quantitative shift in specific activity which changed from 190 in DMSO-activated spores to 260 in heat-activated spores. Autoactivation shifted the increase of β-glucosidase from the time of emergence to 30 minutes before the beginning of the process. DMSO- and urea-induced activation resulted in the accumulation of β-glucosidase activity 15 minutes before emergence. A portion of the trehalase activity of germinating spores was released into the medium regardless which method was used to activate the spores. The β-glucosidase activity of germinating spores was never released into the medium. It is suggested that protein patterns observed during spore germination may not be totally related to the requirements for development but may partially result from the specific activation treatment itself." @default.
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- W1964557478 date "1982-03-01" @default.
- W1964557478 modified "2023-09-27" @default.
- W1964557478 title "Spore-activating agents influence the temporal and quantitative activity of β-glucosidase and trehalase during Dictyostelium discoideum germination" @default.
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- W1964557478 doi "https://doi.org/10.1016/0147-5975(82)90067-6" @default.
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