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• W1964560897 endingPage "151" @default.
• W1964560897 startingPage "147" @default.
• W1964560897 abstract "Abstract A new platform has been developed for DNA lesion detection using a cationic conjugated polymer (CCP). DNA that contains two adjacent thymine bases is irradiated with ultraviolet light to allow for the formation of cyclobutane pyrimidine dimers and pyrimidine–pyrimidone dimers. The DNA lesions block the primer extension, and the base labeled with fluorescein cannot be incorporated into the DNA strand. Addition of the CCP leads to inefficient fluorescence resonance energy transfer (FRET) from CCP to fluorescein. For the case without DNA lesions, successful primer extension allows for efficient FRET between them. In view of the FRET signal changes, the DNA lesions can be detected. This new protocol offers a convenient detection for DNA lesions in aqueous solution without any isolation and washing steps. magnified image" @default.
• W1964560897 created "2016-06-24" @default.
• W1964560897 creator A5011894823 @default.
• W1964560897 creator A5012308909 @default.
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• W1964560897 date "2009-01-22" @default.
• W1964560897 modified "2023-09-30" @default.
• W1964560897 title "Fluorescence-Amplifying Assay for Irradiated DNA Lesions Using Water-Soluble Conjugated Polymers" @default.
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• W1964560897 doi "https://doi.org/10.1002/marc.200800543" @default.
• W1964560897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21706590" @default.
• W1964560897 hasPublicationYear "2009" @default.
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