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• W1964584551 abstract "There are four cysteines (Cys74, Cys104, Cys112 and Cys163) in mature human IL-18 (hIL-18). These cysteines are highly conserved in IL-18s of 11 species cloned so far, suggesting that one or more of the cysteines may be important for hIL-18 function. In this study, each cysteine residue was individually replaced with serine by site-directed mutagenesis. The wild type and mutant IL-18s were expressed in Escherichia coli and renatured by two renaturing methods. The purified wild type and mutant rhIL-18s were assayed for their capacity of inducing IFN-gamma and activating NF-kappaB from ConA-stimulated PBMC. DNA binding activity of NF-kappaB was performed by electrophoretic mobility-shift analysis. Our results showed that the mutant rhIL-18C74S and C163S induced much less amount of IFN-gamma from PBMC and the decrement of NF-kappaB DNA binding activity was also observed from C74S and C163S treated PBMC. These results indicate that functional hIL-18 has an absolute requirement for residues Cys74 and Cys163." @default.
• W1964584551 created "2016-06-24" @default.
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• W1964584551 date "2005-07-01" @default.
• W1964584551 modified "2023-10-18" @default.
• W1964584551 title "Cys74 and Cys163 are necessary for IL-18 to elicit IFN-γ production from peripheral blood lymphoid mononuclear cells" @default.
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• W1964584551 doi "https://doi.org/10.1016/j.molimm.2004.12.013" @default.
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