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- W1964599250 abstract "We characterized the first high-throughput permeability assay platform enabling compound permeability assays, at the full spectrum (1-60dyn/cm <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sup> ) of shear stress, on endothelial cells. The platform comprises four parallel channels, with a porous membrane bonded between layers enabling permeability assays under four shear stresses per chip, ranging ~15x in magnitude. In the bEnd.3 brain endothelial cell line, decreased permeability was observed at rates of 4.06e <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>-8</sup> and 6.04e <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>-8</sup> cm/s per unit shear stress (dyn/cm <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sup> ) for FITC-Dextran and propidium iodide, respectively. Image analysis of cell stains indicated increased elongation and cell alignment with shear stress at rates of 9.15e <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>-4</sup> and 0.12° per dyn/cm <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2</sup> , respectively." @default.
- W1964599250 created "2016-06-24" @default.
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- W1964599250 date "2014-01-01" @default.
- W1964599250 modified "2023-09-27" @default.
- W1964599250 title "A high-throughput permeability assay platform for shear stress characterization of endothelial cells" @default.
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- W1964599250 doi "https://doi.org/10.1109/memsys.2014.6765619" @default.
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