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- W1964619636 abstract "Human enteroviruses are among the most common viruses infecting humans. These viruses are known to be able to infect a wide range of tissues and are believed to establish persistent infections. Enteroviruses are positive-sense single-stranded RNA viruses whose replication involves the synthesis of negative strand intermediates. Therefore, the specific detection of negatively stranded viral RNA in tissues or cells is a reliable marker of active enteroviral replication. The present report presents the development of a real-time RT-PCR allowing the specific detection and quantification of negatively stranded viral RNA. Since it was known that specific amplification of single-stranded RNA can be made difficult by false-priming events leading to false-positive or overestimated results, the assay was developed by using a tagged RT primer. This tagged RT-PCR was shown to be able to amplify specifically negative RNA of enteroviruses grown in cell cultures by preventing the amplification of cDNAs generated by false-priming." @default.
- W1964619636 created "2016-06-24" @default.
- W1964619636 creator A5005772049 @default.
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- W1964619636 creator A5080071788 @default.
- W1964619636 creator A5087138729 @default.
- W1964619636 date "2008-11-01" @default.
- W1964619636 modified "2023-09-27" @default.
- W1964619636 title "Development of a Taqman RT-PCR assay for the detection and quantification of negatively stranded RNA of human enteroviruses: Evidence for false-priming and improvement by tagged RT-PCR" @default.
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- W1964619636 doi "https://doi.org/10.1016/j.jviromet.2008.07.010" @default.
- W1964619636 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18706930" @default.
- W1964619636 hasPublicationYear "2008" @default.
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