FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964677613> ?p ?o ?g. }

• W1964677613 endingPage "141" @default.
• W1964677613 startingPage "133" @default.
• W1964677613 abstract "To understand the mechanism of the maturation of various proteins in protein‐storage vacuoles, we purified a 48‐kDa aspartic endopeptidase composed of 32‐kDa and 16‐kDa subunits from castor bean. Immunocytochemical and cell fractionation analyses of the endosperm of maturing castor bean seed showed that the aspartic endopeptidase was localized in the matrix of the protein‐storage vacuoles, where a variety of seed storage proteins were also present. The amount of the aspartic endopeptidase increased at the mid‐maturation stage of the seeds before accumulation of the storage proteins. To determine how the aspartic endopeptidase is responsible for maturation of seed proteins in concert with the vacuolar processing enzyme, we prepared 35 S‐labeled proproteins of seed proteins from the endoplasmic reticulum fraction of pulse‐labeled maturing endosperm and used the authentic proproteins as substrates for in vitro processing experiments. The purified aspartic endopeptidase was unable to convert any of three endosperm proproteins, pro2S albumin, proglobulin, and proricin, into their mature sizes, while the purified vacuolar processing enzyme could convert all three proproteins. We further examined the activity of aspartic endopeptidase on the cleavage of an internal propeptide of Arabidopsis pro2S albumin, which is known to be removed post‐translationally. The aspartic endopeptidase cleaved the propeptide at three sites under acidic conditions. These results suggest that aspartic endopeptidase cannot directly convert pro2S albumin into the mature form, but it may play a role in trimming the C‐terminal propeptides from the subunits that are produced by the action of the vacuolar processing enzyme." @default.
• W1964677613 created "2016-06-24" @default.
• W1964677613 creator A5028256389 @default.
• W1964677613 creator A5034791415 @default.
• W1964677613 creator A5064120212 @default.
• W1964677613 creator A5084805244 @default.
• W1964677613 date "1997-05-01" @default.
• W1964677613 modified "2023-10-15" @default.
• W1964677613 title "An Aspartic Endopeptidase is Involved in the Breakdown of Propeptides of Storage Proteins in Protein-Storage Vacuoles of Plants" @default.
• W1964677613 cites W1486197790 @default.
• W1964677613 cites W1496647822 @default.
• W1964677613 cites W1509707750 @default.
• W1964677613 cites W1526400750 @default.
• W1964677613 cites W1526745898 @default.
• W1964677613 cites W1533185061 @default.
• W1964677613 cites W1687563194 @default.
• W1964677613 cites W1966323128 @default.
• W1964677613 cites W1975433976 @default.
• W1964677613 cites W1984367265 @default.
• W1964677613 cites W1985101779 @default.
• W1964677613 cites W1988267627 @default.
• W1964677613 cites W2002941671 @default.
• W1964677613 cites W2009817510 @default.
• W1964677613 cites W2010118197 @default.
• W1964677613 cites W2014680794 @default.
• W1964677613 cites W2019587811 @default.
• W1964677613 cites W2021842964 @default.
• W1964677613 cites W2027311860 @default.
• W1964677613 cites W2033322678 @default.
• W1964677613 cites W2034228109 @default.
• W1964677613 cites W2037618997 @default.
• W1964677613 cites W2039999804 @default.
• W1964677613 cites W2054427678 @default.
• W1964677613 cites W2068902682 @default.
• W1964677613 cites W2073472604 @default.
• W1964677613 cites W2086170364 @default.
• W1964677613 cites W2091463834 @default.
• W1964677613 cites W2106013220 @default.
• W1964677613 cites W2108858320 @default.
• W1964677613 cites W2116923223 @default.
• W1964677613 cites W2123775894 @default.
• W1964677613 cites W2133498665 @default.
• W1964677613 cites W2138879032 @default.
• W1964677613 cites W2139691467 @default.
• W1964677613 cites W2169125198 @default.
• W1964677613 cites W2171192142 @default.
• W1964677613 cites W4252620743 @default.
• W1964677613 doi "https://doi.org/10.1111/j.1432-1033.1997.00133.x" @default.
• W1964677613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9210475" @default.
• W1964677613 hasPublicationYear "1997" @default.
• W1964677613 type Work @default.
• W1964677613 sameAs 1964677613 @default.
• W1964677613 citedByCount "103" @default.
• W1964677613 countsByYear W19646776132012 @default.
• W1964677613 countsByYear W19646776132013 @default.
• W1964677613 countsByYear W19646776132014 @default.
• W1964677613 countsByYear W19646776132015 @default.
• W1964677613 countsByYear W19646776132016 @default.
• W1964677613 countsByYear W19646776132017 @default.
• W1964677613 countsByYear W19646776132018 @default.
• W1964677613 countsByYear W19646776132019 @default.
• W1964677613 countsByYear W19646776132020 @default.
• W1964677613 countsByYear W19646776132021 @default.
• W1964677613 countsByYear W19646776132022 @default.
• W1964677613 countsByYear W19646776132023 @default.
• W1964677613 crossrefType "journal-article" @default.
• W1964677613 hasAuthorship W1964677613A5028256389 @default.
• W1964677613 hasAuthorship W1964677613A5034791415 @default.
• W1964677613 hasAuthorship W1964677613A5064120212 @default.
• W1964677613 hasAuthorship W1964677613A5084805244 @default.
• W1964677613 hasConcept C102568950 @default.
• W1964677613 hasConcept C104317684 @default.
• W1964677613 hasConcept C113442657 @default.
• W1964677613 hasConcept C158617107 @default.
• W1964677613 hasConcept C181199279 @default.
• W1964677613 hasConcept C185592680 @default.
• W1964677613 hasConcept C190062978 @default.
• W1964677613 hasConcept C2777774944 @default.
• W1964677613 hasConcept C2781208047 @default.
• W1964677613 hasConcept C515207424 @default.
• W1964677613 hasConcept C55493867 @default.
• W1964677613 hasConcept C86803240 @default.
• W1964677613 hasConcept C97564808 @default.
• W1964677613 hasConceptScore W1964677613C102568950 @default.
• W1964677613 hasConceptScore W1964677613C104317684 @default.
• W1964677613 hasConceptScore W1964677613C113442657 @default.
• W1964677613 hasConceptScore W1964677613C158617107 @default.
• W1964677613 hasConceptScore W1964677613C181199279 @default.
• W1964677613 hasConceptScore W1964677613C185592680 @default.
• W1964677613 hasConceptScore W1964677613C190062978 @default.
• W1964677613 hasConceptScore W1964677613C2777774944 @default.
• W1964677613 hasConceptScore W1964677613C2781208047 @default.
• W1964677613 hasConceptScore W1964677613C515207424 @default.
• W1964677613 hasConceptScore W1964677613C55493867 @default.
• W1964677613 hasConceptScore W1964677613C86803240 @default.
• W1964677613 hasConceptScore W1964677613C97564808 @default.
• W1964677613 hasIssue "1" @default.
• W1964677613 hasLocation W19646776131 @default.

• next