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• W1964685767 abstract "Protein and operon fusions between lacZ and various genes of the nusA.infB operon have been constructed on λ bacteriophages and used to show that the operon is negatively regulated by the level of NusA protein. Overproducing NuSA (but not IF2) from a multicopy plasmid reduces the level of β-galactosidase from the fusions indicating repression of the operon. Introducing the λ carrying the fusions into nusA mutant strains produces a higher level of β-galactosidase-indicative of derepression of the operon. In particular. a larger form of the NusA protein which does not affect bacterial growth per se causes a derepression of the operon. As both protein and operon fusions respond equivalently, we conclude that the nusA protein is acting at the transcriptional level to regulate expression of the nusA . infB operon." @default.
• W1964685767 created "2016-06-24" @default.
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• W1964685767 date "1985-01-01" @default.
• W1964685767 modified "2023-10-03" @default.
• W1964685767 title "Effect of NusA protein on expression of the<i>nusA, infB</i>operon in<i>E.coli</i>" @default.
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• W1964685767 doi "https://doi.org/10.1093/nar/13.9.3371" @default.
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