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- W1964696759 abstract "Abstract The development of a modified electrode for the selective determination of p-nitrophenol (PNP) to be applied in the determination of the enzymatic activity (EA) of acid phosphatase using p-nitrophenylphosphate (PNPP) as substrate is described. Elimination of the interference of PNPP was made by covering the electrode surface using 25 μl of a 5% (m/v) Nafion® solution. In comparison to the glassy carbon (GC) electrode without modification, the presence of the membrane promoted a reduction in the sensitivity and an increase in the detection limit for the determination of PNP, in addition to a decrease of the electron transfer constant. The GC electrode covered with a Nafion® membrane showed a linear response range between 20 and 230 μmol l−1 for PNP, adjusted by the equation IP=0.157+0.0089 [PNP], for N=22 with a correlation coefficient of 0.9984. This electrode was used for the determination of the EA of acid phosphatase from castor bean seeds and an activity of 38.40 U mg−1 was found." @default.
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- W1964696759 date "2001-08-01" @default.
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- W1964696759 title "Electroanalytical determination of acid phosphatase activity by monitoring p-nitrophenol" @default.
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- W1964696759 doi "https://doi.org/10.1016/s0003-2670(01)01120-5" @default.
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