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• W1964727104 startingPage "507" @default.
• W1964727104 abstract "Archaeal DNA repair pathways are not well defined; in particular, there are no convincing candidate proteins for detection of DNA mismatches or the bulky lesions removed by excision repair pathways. Single-stranded DNA-binding proteins (SSBs) play a central role in DNA replication, recombination and repair. The crenarchaeal SSB is a monomer with a single oligonucleotide-binding fold for single-stranded DNA binding coupled to a flexible C-terminal tail reminiscent of bacterial SSB that mediates interactions with other proteins. We demonstrate that Sulfolobus solfataricus SSB can melt DNA containing a mismatch or DNA lesion specifically in vitro. We suggest that a potential role for SSB in archaea is the detection of DNA damage due to local destabilisation of the DNA double helix, followed by recruitment of specific repair proteins. Proteins interacting specifically with a single-stranded DNA:SSB complex include several known or putative DNA repair proteins and DNA helicases." @default.
• W1964727104 created "2016-06-24" @default.
• W1964727104 creator A5023338935 @default.
• W1964727104 creator A5057021821 @default.
• W1964727104 date "2005-10-01" @default.
• W1964727104 modified "2023-09-27" @default.
• W1964727104 title "DNA Damage Detection by an Archaeal Single-stranded DNA-binding Protein" @default.
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• W1964727104 doi "https://doi.org/10.1016/j.jmb.2005.08.050" @default.
• W1964727104 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16181640" @default.
• W1964727104 hasPublicationYear "2005" @default.
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