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• W1964730147 abstract "Abstract CD56 bright natural killer (NK) cells, generated in vitro from CD34 + hematopoietic progenitor cells, were characterized after a 30‐day culture with flt3 ligand plus IL‐15. Virtually, all CD56 bright cells expressed CD117, CD25, natural cytotoxicity receptors (NCRs), NKG2D, CD161, and CD244, while only a subset expressed CD18‐CD11a (LFA‐1), and CD94 molecule, defining an immature CD56 bright /NCRs + /NKG2D + /LFA‐1 − /CD94 − subset. Another small subset of cells expressing CD94 but not LFA‐1 integrin was also identified, suggesting that during NK differentiation LFA‐1 might be upregulated later than CD94. To verify this hypothesis in vivo, we evaluated the NK cell expression of LFA‐1 in both peripheral and umbilical cord blood samples. Interestingly, in these blood fluids, we have identified a lineage negative CD34 − /LFA‐1 low /NKp46 dim /NKG2D dim /CD94 − subset that resembled an immature stage of NK cells present in lymph nodes. Altogether, the results indicate that CD18‐CD11a integrin, as well as CD11b in mice, may be a useful marker to identify immature stages of NK cell differentiation. © 2009 International Society for Advancement of Cytometry" @default.
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• W1964730147 date "2009-09-09" @default.
• W1964730147 modified "2023-10-17" @default.
• W1964730147 title "Identification of a NCR⁺/NKG2D⁺/LFA-1^low/CD94⁻immature human NK cell subset" @default.
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• W1964730147 doi "https://doi.org/10.1002/cyto.a.20789" @default.
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