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• W1964750756 abstract "Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis." @default.
• W1964750756 created "2016-06-24" @default.
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• W1964750756 date "1991-12-15" @default.
• W1964750756 modified "2023-09-27" @default.
• W1964750756 title "Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis." @default.
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• W1964750756 doi "https://doi.org/10.1073/pnas.88.24.11388" @default.
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