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- W1964760103 abstract "Single-cell suspensions of adult lizard (Chalcides ocellatus) spleen have been induced, in vitro, to produce a primary immune response. Using rat red cells (RRBC) as antigen and the culture conditions normally used in most vertebrate species but new for reptilia, it has been found that, in vitro at 37°C, lizard spleen cells produce an antibody-forming response optimal at day 10. The response depends on the number of cultured cells and the dose of antigen, and parallels that obtained in vivo. Leibovitz (L-15) medium supplemented with 10% normal adult lizard serum was a satisfactory culture medium. 2-mercaptoethanol (2-ME), an ingredient used in mammalian cell culture, enhanced antibody production in lizard cells." @default.
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- W1964760103 date "1988-06-01" @default.
- W1964760103 modified "2023-09-23" @default.
- W1964760103 title "Primary in vitro stimulation of antibody production by lizard splenocytes" @default.
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- W1964760103 doi "https://doi.org/10.1016/0165-2427(88)90163-8" @default.
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