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• W1964793873 abstract "Four methods for measuring diffusion coefficients were compared on a microfabricated fluidic device using rhodamine 6G as the analyte. The measurements were made using a static imaging method and three dynamic methods—stopped flow, varying the applied potential (E-field method), and varying the detection length (length method). Under conditions where analyte–wall interactions (adsorption) are minimized, e.g. in a 50/50 (v/v) methanol/aqueous buffer, the stopped flow (2.71±0.09×10−6 cm2 s−1), E-field (2.684±0.005×10−6 cm2 s−1) and the static imaging (2.69±0.02×10−6 cm2 s−1) measurements were all within experimental error of one another and previously reported values. Under 100% aqueous conditions, however, the diffusion coefficient measured dynamically was 11% larger than that measured statically. Diffusion coefficients for rhodamine B, fluorescein, 2′,7′dichloro-fluorescein (DCF), rhodamine 6G, tetramethylrhodamine labeled glutamic acid and isoleucine, and fluorescein conjugated bovine serum albumin and ovalbumin were also measured using the static imaging method." @default.
• W1964793873 created "2016-06-24" @default.
• W1964793873 creator A5081232411 @default.
• W1964793873 date "2002-02-11" @default.
• W1964793873 modified "2023-10-12" @default.
• W1964793873 title "Diffusion coefficient measurements in microfluidic devices" @default.
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• W1964793873 doi "https://doi.org/10.1016/s0039-9140(01)00602-6" @default.
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