Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964865601> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1964865601 endingPage "3660" @default.
- W1964865601 startingPage "3653" @default.
- W1964865601 abstract "The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity electrophoresis system based on Phenylmercuric substituted polyacrylamide (APM) gels allows separation of the RNA chains as a function of their s4U content. Distribution of s4U within chains follows a binomial law indicating that (i) substitution is close to random, (ii) efficiency of s4U incorporation is 0.22 times that of U. The monothiolated RNA fraction isolated from APM gel was irradiated at 366 nm under native conditions and the intramolecularly crosslinked molecules, (34%), were separated on denaturing polyacrylamide gel according to loop size. The positions of the two partners of bridges were identified by mean of reverse transcription and RNA sequencing. 17 of the 41 possible s4U positions lead to detectable bridges. These crosslinks formed efficiently at the border of bihelical regions or when structural mobility is allowed. The pattern of crosslinks is in agreement with the previously proposed secondary structure but indicates that it is much more flexible than expected." @default.
- W1964865601 created "2016-06-24" @default.
- W1964865601 creator A5013093254 @default.
- W1964865601 creator A5066289297 @default.
- W1964865601 creator A5082857363 @default.
- W1964865601 date "1991-01-01" @default.
- W1964865601 modified "2023-09-30" @default.
- W1964865601 title "Conformation and structural fluctuations of a 218 nucleotides long rRNA fragment: 4-thiouridine as an intrinsic photolabelling probe" @default.
- W1964865601 cites W1039907071 @default.
- W1964865601 cites W1490565589 @default.
- W1964865601 cites W1564666527 @default.
- W1964865601 cites W1593832498 @default.
- W1964865601 cites W1963774448 @default.
- W1964865601 cites W1963899534 @default.
- W1964865601 cites W1967977918 @default.
- W1964865601 cites W1981898142 @default.
- W1964865601 cites W1999434093 @default.
- W1964865601 cites W2001333672 @default.
- W1964865601 cites W2017177614 @default.
- W1964865601 cites W2025401091 @default.
- W1964865601 cites W2026358434 @default.
- W1964865601 cites W2048438965 @default.
- W1964865601 cites W2055941888 @default.
- W1964865601 cites W2091022497 @default.
- W1964865601 cites W2095239103 @default.
- W1964865601 cites W2141728252 @default.
- W1964865601 doi "https://doi.org/10.1093/nar/19.13.3653" @default.
- W1964865601 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/328394" @default.
- W1964865601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1712940" @default.
- W1964865601 hasPublicationYear "1991" @default.
- W1964865601 type Work @default.
- W1964865601 sameAs 1964865601 @default.
- W1964865601 citedByCount "50" @default.
- W1964865601 countsByYear W19648656012013 @default.
- W1964865601 countsByYear W19648656012017 @default.
- W1964865601 countsByYear W19648656012021 @default.
- W1964865601 countsByYear W19648656012022 @default.
- W1964865601 crossrefType "journal-article" @default.
- W1964865601 hasAuthorship W1964865601A5013093254 @default.
- W1964865601 hasAuthorship W1964865601A5066289297 @default.
- W1964865601 hasAuthorship W1964865601A5082857363 @default.
- W1964865601 hasBestOaLocation W19648656012 @default.
- W1964865601 hasConcept C101660505 @default.
- W1964865601 hasConcept C104317684 @default.
- W1964865601 hasConcept C116084860 @default.
- W1964865601 hasConcept C12554922 @default.
- W1964865601 hasConcept C138885662 @default.
- W1964865601 hasConcept C153911025 @default.
- W1964865601 hasConcept C179926584 @default.
- W1964865601 hasConcept C181199279 @default.
- W1964865601 hasConcept C2776449523 @default.
- W1964865601 hasConcept C2778862431 @default.
- W1964865601 hasConcept C40250595 @default.
- W1964865601 hasConcept C41895202 @default.
- W1964865601 hasConcept C512185932 @default.
- W1964865601 hasConcept C55493867 @default.
- W1964865601 hasConcept C67705224 @default.
- W1964865601 hasConcept C67905577 @default.
- W1964865601 hasConcept C86803240 @default.
- W1964865601 hasConceptScore W1964865601C101660505 @default.
- W1964865601 hasConceptScore W1964865601C104317684 @default.
- W1964865601 hasConceptScore W1964865601C116084860 @default.
- W1964865601 hasConceptScore W1964865601C12554922 @default.
- W1964865601 hasConceptScore W1964865601C138885662 @default.
- W1964865601 hasConceptScore W1964865601C153911025 @default.
- W1964865601 hasConceptScore W1964865601C179926584 @default.
- W1964865601 hasConceptScore W1964865601C181199279 @default.
- W1964865601 hasConceptScore W1964865601C2776449523 @default.
- W1964865601 hasConceptScore W1964865601C2778862431 @default.
- W1964865601 hasConceptScore W1964865601C40250595 @default.
- W1964865601 hasConceptScore W1964865601C41895202 @default.
- W1964865601 hasConceptScore W1964865601C512185932 @default.
- W1964865601 hasConceptScore W1964865601C55493867 @default.
- W1964865601 hasConceptScore W1964865601C67705224 @default.
- W1964865601 hasConceptScore W1964865601C67905577 @default.
- W1964865601 hasConceptScore W1964865601C86803240 @default.
- W1964865601 hasIssue "13" @default.
- W1964865601 hasLocation W19648656011 @default.
- W1964865601 hasLocation W19648656012 @default.
- W1964865601 hasLocation W19648656013 @default.
- W1964865601 hasLocation W19648656014 @default.
- W1964865601 hasOpenAccess W1964865601 @default.
- W1964865601 hasPrimaryLocation W19648656011 @default.
- W1964865601 hasRelatedWork W1139788748 @default.
- W1964865601 hasRelatedWork W1982270490 @default.
- W1964865601 hasRelatedWork W1994709864 @default.
- W1964865601 hasRelatedWork W1999076623 @default.
- W1964865601 hasRelatedWork W2038266014 @default.
- W1964865601 hasRelatedWork W2091718956 @default.
- W1964865601 hasRelatedWork W2134951665 @default.
- W1964865601 hasRelatedWork W2373058508 @default.
- W1964865601 hasRelatedWork W4235238160 @default.
- W1964865601 hasRelatedWork W82316114 @default.
- W1964865601 hasVolume "19" @default.
- W1964865601 isParatext "false" @default.
- W1964865601 isRetracted "false" @default.
- W1964865601 magId "1964865601" @default.
- W1964865601 workType "article" @default.