FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1964878787> ?p ?o ?g. }

• W1964878787 endingPage "215" @default.
• W1964878787 startingPage "209" @default.
• W1964878787 abstract "Objectives Despite their distinct biology, granulosa cell tumours (GCTs) are treated similarly to other ovarian tumours. Predominantly expressed in granulosa cells, the transcription factor Forkhead Box L2 (FOXL2) is near absent in juvenile-type GCTs. This research aimed to investigate miRNAs as a mechanism of suppression of FOXL2 expression in juvenile-type GCTs. Methods The miRNA abundance of two GCT cell lines COV434 and KGN was profiled using Affymetrix miRNA GeneChip arrays. Luciferase assays were used to confirm miRNA binding to the 3′UTR of FOXL2. Identified as promising candidates, the miR-17 miRNA family was targeted for knockdown with a miRNA sponge. Additionally, individual family members miR-17, miR-20b and miR-106a were knocked down using Anti-miR™ inhibitors. Subsequently, FOXL2 expression was analysed using RT-qPCR and Western blotting. Results The profiling of COV434 and KGN cells revealed unique miRNA signatures, with COV434 expressing miR-17 family miRNAs whilst KGN expressed members of the let-7 miRNA gene family. Luciferase assays confirmed miRNA binding to FOXL2's 3′UTR. Reduction of miR-17 family miRNAs increased FOXL2 mRNA expression, however luciferase assays performed in combination with the sponge suggested this is an indirect effect. As no changes in protein were observed, we propose another miRNA is repressing the translation of FOXL2 mRNA. Conclusion Through miRNA profiling we have begun to unravel the profiles of GCTs, showing that juvenile and adult derived-cell lines are biologically distinct. By expanding on this discovery we may further elucidate the miRNA–mRNA pathways involved in GCT initiation and progression with potential for novel therapeutics for these cancers." @default.
• W1964878787 created "2016-06-24" @default.
• W1964878787 creator A5011618878 @default.
• W1964878787 creator A5032506037 @default.
• W1964878787 creator A5049860411 @default.
• W1964878787 date "2013-04-01" @default.
• W1964878787 modified "2023-09-25" @default.
• W1964878787 title "Comparative study of microRNA regulation on FOXL2 between adult-type and juvenile-type granulosa cell tumours in vitro" @default.
• W1964878787 cites W1577577364 @default.
• W1964878787 cites W1972989638 @default.
• W1964878787 cites W1973318991 @default.
• W1964878787 cites W1973816411 @default.
• W1964878787 cites W1976722903 @default.
• W1964878787 cites W1984935494 @default.
• W1964878787 cites W1987544972 @default.
• W1964878787 cites W1993800649 @default.
• W1964878787 cites W1998734533 @default.
• W1964878787 cites W2013881488 @default.
• W1964878787 cites W2020656583 @default.
• W1964878787 cites W2021528515 @default.
• W1964878787 cites W2029872286 @default.
• W1964878787 cites W2031014255 @default.
• W1964878787 cites W2031252637 @default.
• W1964878787 cites W2032457040 @default.
• W1964878787 cites W2056616628 @default.
• W1964878787 cites W205885735 @default.
• W1964878787 cites W2061564388 @default.
• W1964878787 cites W2062290101 @default.
• W1964878787 cites W2066351138 @default.
• W1964878787 cites W2066801022 @default.
• W1964878787 cites W2083381199 @default.
• W1964878787 cites W2090396315 @default.
• W1964878787 cites W2098159000 @default.
• W1964878787 cites W2102876017 @default.
• W1964878787 cites W2106381149 @default.
• W1964878787 cites W2106876536 @default.
• W1964878787 cites W2108836814 @default.
• W1964878787 cites W2110969580 @default.
• W1964878787 cites W2116799486 @default.
• W1964878787 cites W2125536613 @default.
• W1964878787 cites W2129414453 @default.
• W1964878787 cites W2133464976 @default.
• W1964878787 cites W2138165502 @default.
• W1964878787 cites W2140253494 @default.
• W1964878787 cites W2141157874 @default.
• W1964878787 cites W2142583406 @default.
• W1964878787 cites W2143338249 @default.
• W1964878787 cites W2144971212 @default.
• W1964878787 cites W2149548143 @default.
• W1964878787 cites W2151535331 @default.
• W1964878787 cites W2154570280 @default.
• W1964878787 cites W2162674813 @default.
• W1964878787 cites W2910065277 @default.
• W1964878787 cites W4237473415 @default.
• W1964878787 cites W4378618906 @default.
• W1964878787 cites W57303695 @default.
• W1964878787 cites W3145571634 @default.
• W1964878787 doi "https://doi.org/10.1016/j.ygyno.2012.12.034" @default.
• W1964878787 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23280087" @default.
• W1964878787 hasPublicationYear "2013" @default.
• W1964878787 type Work @default.
• W1964878787 sameAs 1964878787 @default.
• W1964878787 citedByCount "13" @default.
• W1964878787 countsByYear W19648787872013 @default.
• W1964878787 countsByYear W19648787872016 @default.
• W1964878787 countsByYear W19648787872017 @default.
• W1964878787 countsByYear W19648787872018 @default.
• W1964878787 countsByYear W19648787872021 @default.
• W1964878787 countsByYear W19648787872022 @default.
• W1964878787 countsByYear W19648787872023 @default.
• W1964878787 crossrefType "journal-article" @default.
• W1964878787 hasAuthorship W1964878787A5011618878 @default.
• W1964878787 hasAuthorship W1964878787A5032506037 @default.
• W1964878787 hasAuthorship W1964878787A5049860411 @default.
• W1964878787 hasConcept C104317684 @default.
• W1964878787 hasConcept C105580179 @default.
• W1964878787 hasConcept C111335760 @default.
• W1964878787 hasConcept C145059251 @default.
• W1964878787 hasConcept C150194340 @default.
• W1964878787 hasConcept C153911025 @default.
• W1964878787 hasConcept C173396325 @default.
• W1964878787 hasConcept C18431079 @default.
• W1964878787 hasConcept C22667442 @default.
• W1964878787 hasConcept C502942594 @default.
• W1964878787 hasConcept C54009773 @default.
• W1964878787 hasConcept C54355233 @default.
• W1964878787 hasConcept C81885089 @default.
• W1964878787 hasConcept C86803240 @default.
• W1964878787 hasConcept C89604277 @default.
• W1964878787 hasConcept C95444343 @default.
• W1964878787 hasConceptScore W1964878787C104317684 @default.
• W1964878787 hasConceptScore W1964878787C105580179 @default.
• W1964878787 hasConceptScore W1964878787C111335760 @default.
• W1964878787 hasConceptScore W1964878787C145059251 @default.
• W1964878787 hasConceptScore W1964878787C150194340 @default.
• W1964878787 hasConceptScore W1964878787C153911025 @default.
• W1964878787 hasConceptScore W1964878787C173396325 @default.
• W1964878787 hasConceptScore W1964878787C18431079 @default.

• next