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• W1964972974 endingPage "17366" @default.
• W1964972974 startingPage "17350" @default.
• W1964972974 abstract "The effects of hypoxia and perfluorooctane sulfonamide (PFOSA), given singly and also in combination on endocrine, biotransformation, and oxidative stress responses were investigated in primary culture of salmon hepatocytes. Hypoxia was induced chemically using cobalt chloride (CoCl2) or deferroxamine (DFO). Primary culture of salmon hepatocytes were exposed to either CoCl2 (150 μM) or DFO (100 μM), in the presence or absence of PFOSA at 0, 25, and 50 μM for 24 and 48 h. Changes in transcript levels were analyzed by quantitative (real-time) PCR using gene-specific primers. CYP, catalase, GST, and SOD activities were analyzed spectrophotometrically. The hif-1α mRNA was used to validate cellular hypoxic condition, showing significantly induced transcription after 48-h exposure to DFO and CoCl2. Our data show that transcript levels for endocrine (ERα, Vtg, and Zrp), biotransformation (cyp1a, cyp3a, gst, and udpgt), and oxidative stress responses (catalase (cat), glutathione peroxidase (gpx), and glutathione reductase (gr)) were differentially modulated by PFOSA and hypoxia alone, and these effects were dependent on the response parameters and time of exposure. In combined exposure scenarios, the observed effects were apparently hypoxia-dependent. However, the observed effects at transcript levels were not concomitant with those at functional protein levels, further emphasizing the potential differences that may exist between these biological levels. Biplot of principal component analysis (PCA) showed grouping of response variables after 48 h of exposure. The distribution of observations and variables indicate that PFOSA had little effect on most response variables, while clustering show a unique association between a given hypoxia condition (i.e., CoCl2 or DFO) in combination with PFOSA and transcripts, proteins, or enzyme activities." @default.
• W1964972974 created "2016-06-24" @default.
• W1964972974 creator A5075301522 @default.
• W1964972974 creator A5085320289 @default.
• W1964972974 date "2014-12-04" @default.
• W1964972974 modified "2023-09-27" @default.
• W1964972974 title "Endocrine, biotransformation, and oxidative stress responses in salmon hepatocytes exposed to chemically induced hypoxia and perfluorooctane sulfonamide (PFOSA), given singly or in combination" @default.
• W1964972974 cites W1484675482 @default.
• W1964972974 cites W149914726 @default.
• W1964972974 cites W1581414347 @default.
• W1964972974 cites W1591278389 @default.
• W1964972974 cites W1964542762 @default.
• W1964972974 cites W1966308151 @default.
• W1964972974 cites W1966490148 @default.
• W1964972974 cites W1968124397 @default.
• W1964972974 cites W1969655062 @default.
• W1964972974 cites W1969817100 @default.
• W1964972974 cites W1974095899 @default.
• W1964972974 cites W1976770880 @default.
• W1964972974 cites W1977229794 @default.
• W1964972974 cites W1979209667 @default.
• W1964972974 cites W1980138755 @default.
• W1964972974 cites W1985421585 @default.
• W1964972974 cites W1988695122 @default.
• W1964972974 cites W1989226681 @default.
• W1964972974 cites W1990519331 @default.
• W1964972974 cites W1993586084 @default.
• W1964972974 cites W1998832327 @default.
• W1964972974 cites W2003525539 @default.
• W1964972974 cites W2006998778 @default.
• W1964972974 cites W2011610363 @default.
• W1964972974 cites W2011840450 @default.
• W1964972974 cites W2012702119 @default.
• W1964972974 cites W2012959131 @default.
• W1964972974 cites W2014860595 @default.
• W1964972974 cites W2015454673 @default.
• W1964972974 cites W2017750872 @default.
• W1964972974 cites W2020053289 @default.
• W1964972974 cites W2020469902 @default.
• W1964972974 cites W2020759392 @default.
• W1964972974 cites W2021741218 @default.
• W1964972974 cites W2023364983 @default.
• W1964972974 cites W2025509105 @default.
• W1964972974 cites W2029243892 @default.
• W1964972974 cites W2030384319 @default.
• W1964972974 cites W2030434933 @default.
• W1964972974 cites W2030676023 @default.
• W1964972974 cites W2031165634 @default.
• W1964972974 cites W2033008296 @default.
• W1964972974 cites W2033900299 @default.
• W1964972974 cites W2038231323 @default.
• W1964972974 cites W2038656521 @default.
• W1964972974 cites W2038691917 @default.
• W1964972974 cites W2043875467 @default.
• W1964972974 cites W2045863819 @default.
• W1964972974 cites W2046262374 @default.
• W1964972974 cites W2052097318 @default.
• W1964972974 cites W2053656714 @default.
• W1964972974 cites W2057310849 @default.
• W1964972974 cites W2057573011 @default.
• W1964972974 cites W2059328741 @default.
• W1964972974 cites W2060330947 @default.
• W1964972974 cites W2061478139 @default.
• W1964972974 cites W2062379585 @default.
• W1964972974 cites W2063029924 @default.
• W1964972974 cites W2064507515 @default.
• W1964972974 cites W2067834800 @default.
• W1964972974 cites W2069178739 @default.
• W1964972974 cites W2070235651 @default.
• W1964972974 cites W2074630416 @default.
• W1964972974 cites W2074882369 @default.
• W1964972974 cites W2077104587 @default.
• W1964972974 cites W2082540577 @default.
• W1964972974 cites W2083135851 @default.
• W1964972974 cites W2084522082 @default.
• W1964972974 cites W2085470258 @default.
• W1964972974 cites W2087040133 @default.
• W1964972974 cites W2091051585 @default.
• W1964972974 cites W2094908805 @default.
• W1964972974 cites W2097348395 @default.
• W1964972974 cites W2098909342 @default.
• W1964972974 cites W2104124411 @default.
• W1964972974 cites W2108115312 @default.
• W1964972974 cites W2108742374 @default.
• W1964972974 cites W2109437527 @default.
• W1964972974 cites W2114422046 @default.
• W1964972974 cites W2123300316 @default.
• W1964972974 cites W2124274165 @default.
• W1964972974 cites W2125202234 @default.
• W1964972974 cites W2128635872 @default.
• W1964972974 cites W2128894493 @default.
• W1964972974 cites W2131699244 @default.
• W1964972974 cites W2132066544 @default.
• W1964972974 cites W2132927623 @default.
• W1964972974 cites W2136973735 @default.
• W1964972974 cites W2137671292 @default.
• W1964972974 cites W2140858853 @default.
• W1964972974 cites W2144062280 @default.

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