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- W1965036914 abstract "Esterase from thermophilic bacteria Alicyclobacillus acidocaldarius can be produced up to 200 μg/ml by coupled in vitro transcription/translation system derived from Escherichia coli. The synthesized thermostable enzyme can be determined by photometrical and fluorescent assays at least up to 10 −8 M concentration or by activity staining in the polyacrylamide gels. Enhanced green fluorescence protein‐esterase fusion protein was bound to a matrix with immobilized esterase inhibitor and purified by affinity chromatography. Thus, the esterase is suited as a reporter enzyme to monitor the expression of polypeptides coupled to its N‐terminus and simultaneously, as a cleavable tag for polypeptide purification." @default.
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- W1965036914 date "2005-03-10" @default.
- W1965036914 modified "2023-10-13" @default.
- W1965036914 title "The esterase from<i>Alicyclobacillus acidocaldarius</i>as a reporter enzyme and affinity tag for protein biosynthesis" @default.
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- W1965036914 doi "https://doi.org/10.1016/j.febslet.2005.02.059" @default.
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