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- W1965053377 abstract "Abstract A new analytical method (two-wavelength/double beam method) for identification of respiratory-chain components in mitochondria was developed using a commercially available two-wavelength spectrophotometer. Cancellation of the scattered light due to the turbidity of the sample and correction of baseline due to the errors in the appartus were achieved using an optical attenuator for the reference cuvette over a wide range of wavelength ( λ x ) taking λ 1 as the reference wavelength. Then the same beams were passed through the saple cuvette and the difference between the absorbance of the sample and reference cuvettes at λ x was obtained. Difference spectra between reduced and oxidized rat-liver mitochondria were obtained on a full scale of A 0–0.01 or 0.03 in varying wavelengths of 300–650 nm. Using this method the α-bands of cytochrome a (+ a 3 ), b and c , the Soret bands of cytochrome a 3 (+ a ) and b , and the bands of flavoprotein and pyridine nucleotides were detected in a quite sensitive manner with reasonable accuracy. A discussion is given on the theory and application of this method." @default.
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- W1965053377 date "1972-05-01" @default.
- W1965053377 modified "2023-09-27" @default.
- W1965053377 title "New sensitive method for the spectral analysis of respiratory-chain components in mitochondria" @default.
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- W1965053377 doi "https://doi.org/10.1016/0005-2728(72)90117-x" @default.
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